TAILIEUCHUNG - Báo cáo khoa học: The F13 residue is critical for interaction among the coat protein subunits of papaya mosaic virus

Papaya mosaic virus (PapMV) coat protein (CP) inEscherichia coliwas previously showed to self-assemble in nucleocapsid-like particles (NLPs) that were similar in shape and appearance to the native virus. We have also shown that a truncated CP missing the N-terminal 26 amino acids is mono-meric and loses its ability to bind RNA. | ễFEBS Journal The F13 residue is critical for interaction among the coat protein subunits of papaya mosaic virus M. E. Laliberte Gagne1 K. Lecours2 S. Gagne2 and D. Leclerc1 1 Infectious Disease Research Centre LavalUniversity Quebec Canada 2 Department of Biochemistry LavalUniversity Quebec Canada Keywords Nucleocapsid-like particles NLPs PapMV papaya mosaic virus potexvirus virus self-assembly Correspondence D. Leclerc Infectious Disease Research Centre LavalUniversity QC Canada Fax 1 418 654 2715 Tel 1 418 654 2705 E-mail Received 31 August 2007 revised 6 December 2007 accepted 21 January 2008 doi Papaya mosaic virus PapMV coat protein CP in Escherichia coli was previously showed to self-assemble in nucleocapsid-like particles NLPs that were similar in shape and appearance to the native virus. We have also shown that a truncated CP missing the N-terminal 26 amino acids is monomeric and loses its ability to bind RNA. It is likely that the N-terminus of the CP is important for the interaction between the subunits in self-assembly into NLPs. In this work through deletion and mutation analysis we have shown that the deletion of 13 amino acids is sufficient to generate the monomeric form of the CP. Furthermore we have shown that residue F13 is critical for self-assembly of the CP subunits into NLPs. The replacement of F13 with hydrophobic residues L or Y generated mutated forms of the CP that were able to self-assemble into NLPs. However the replacement of F13 by A G R E or S was detrimental to the self-assembly of the protein into NLPs. We concluded that a hydrophobic interaction at the N-ter-minus is important to ensure self-assembly of the protein into NLPs. We also discuss the importance of F13 for assembly of other members of the potexvirus family. Papaya mosaic virus PapMV is a member of the potexvirus family. Its virion is a flexuous rod that is 500 nm long and 13 nm in diameter. A PapMV particle is .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• molecular Biology
• tumor cells
• Antimicrobial
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• biochemical studies
• environmental medicine