TAILIEUCHUNG - Báo cáo khoa học: Transduced human PEP-1–heat shock protein 27 efficiently protects against brain ischemic insult

Reactive oxygen species contribute to the development of various human diseases. Ischemia is characterized by both significant oxidative stress and characteristic changes in the antioxidant defense mechanism. Heat shock protein 27 (HSP27) has a potent ability to increase cell survival in response to oxidative stress. In the present study, we have investigated the protective effects of PEP-1–HSP27 against cell death and ischemic insults. | ễFEBS Journal Transduced human PEP-1-heat shock protein 27 efficiently protects against brain ischemic insult Jae J. An1 z Yeom P. Lee1 z So Y. Kim1 Sun H. Lee1 Min J. Lee1 Min S. Jeong1 Dae W. Kim1 Sang H. Jang1 Ki-Yeon Yoo2 Moo H. Won2 Tae-Cheon Kang2 Oh-Shin Kwon3 Sung-Woo Cho4 Kil S. Lee1 Jinseu Park1 Won S. Eum1 and Soo Y. Choi1 1 Department of BiomedicalScience and Research Institute for Bioscience and Biotechnology Hallym University Chunchon Korea 2 Department of Anatomy and Neurobiology College of Medicine Hallym University Chunchon Korea 3 Department of Biochemistry Kyungpook NationalUniversity Taegu Korea 4 Department of Biochemistry and Molecular Biology University of Ulsan College of Medicine Seoul Korea Keywords heat shock protein 27 ischemia protein therapy protein transduction ROS Correspondence S. Y. Choi Department of Biomedical Science and Research Institute for Bioscience and Biotechnology Hallym University Chunchon 200-702 Korea Fax 82 33 241 1463 Tel 82 33 248 2112 E-mail sychoi@ W. S. Eum Department of Biomedical Science and Research Institute for Bioscience and Biotechnology Hallym University Chunchon 200-702 Korea Fax 82 33 241 1463 Tel 82 33 248 2112 E-mail wseum@ Reactive oxygen species contribute to the development of various human diseases. Ischemia is characterized by both significant oxidative stress and characteristic changes in the antioxidant defense mechanism. Heat shock protein 27 HSP27 has a potent ability to increase cell survival in response to oxidative stress. In the present study we have investigated the protective effects of PEP-1-HSP27 against cell death and ischemic insults. When PEP-1-HSP27 fusion protein was added to the culture medium of astrocyte and primary neuronal cells it rapidly entered the cells and protected them against cell death induced by oxidative stress. Immunohistochemical analysis revealed that when PEP-1-HSP27 fusion protein was intraperitoneally injected into gerbils it .

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