TAILIEUCHUNG - Báo cáo lâm nghiệp: "Chromosomal differentiation between Pinus heldreichii and Pinus nigra"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài:"Chromosomal differentiation between Pinus heldreichii and Pinus nigra" | Ann. For. Sci. 63 2006 267- 274 INRA EDP Sciences 2006 DOI forest 2006005 267 Original article Chromosomal differentiation between Pinus heldreichii and Pinus nigra Faruk BoGUNiCa Edina MURATOViCb Sonja Siljak-Yakovlevc a University of Sarajevo Faculty of Forestry Zagrebacka 20 71000 Sarajevo Bosnia and Herzegovina b University of Sarajevo Faculty of Sciences Department of Biology Laboratory for research and protection of endemic resources Zmaja od Bosne 33 71000 Sarajevo Bosnia and Herzegovina c Université Paris-Sud UMR CNRS 8079 Écologie Systématique Évolution Bât. 360 91405 Orsay France Received 17 October 2004 accepted 12 July 2005 Abstract - Two tertiary relict pines Pinus heldreichii and P. nigra have been considered as taxonomically very close species. Both species have very discontinuous geographical distributions which overlap in some localities in Balkan Peninsula. The pattern of heterochromatin regions distribution AT- GC-rich and DAPI positive heterochromatin and activity of nucleolar organizing regions NORs were analyzed for these two pines by fluorochrome banding and silver staining respectively. Morphometric data for karyotype of P. heldreichii were presented here for the first time. Chromomycin CMA banding pattern was particularly species specific P. heldreichii possessed 12 and P. nigra 24 bands. In comparison with other species from subsection Pinus P. heldreichii displayed particular CMA-banding pattern. Hoechst banding was less specific and similar to other pines. DAPI staining was applied after DNA denaturation renaturation and revealed important differences in number and position of bands between the two species. Number of secondary constrictions SCs of NORs and of nucleoli also differed between P. heldreichii 10 and P. nigra 12 . Our results proved a clear interspecific differentiation at the chromosomal level between the two species and add some data to discuss about the possible new subsectional placement of P. heldreichii. Pinus .

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