TAILIEUCHUNG - Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells

The specific features of the plasticity of adult stem cells are largely unknown. Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs). To identify the genes responsible for hepatic differentiation, we examined the gene expres-sion profiles of AT-MSC-derived hepatocytes (AT-MSC-Hepa) using several microarray methods. | ễFEBS Journal A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells Yusuke Yamamoto1 2 Agnieszka Banas1 z Shigenori Murata3 Madoka Ishikawa3 Chun R. Lim3 Takumi Teratani1 Izuho Hatada4 Kenichi Matsubara3 Takashi Kato2 and Takahiro Ochiya1 2 1 Section for Studies on Metastasis NationalCancer Center Research Institute Tokyo Japan 2 Graduate Schoolof Science and Engineering Waseda University Tokyo Japan 3 DNA Chip Research Inc. Yokohama Japan 4 Laboratory of Genome Science BiosignalGenome Resource Center Department of Molecular and Cellular Biology Gunma University Maebashi Japan Keywords adipose tissue gene ontology hepatocyte differentiation mesenchymal stem cell microarray Correspondence T. Ochiya Section for Studies on Metastasis National Cancer Center Research Institute 1-1 Tsukiji 5-chome Chuo-ku Tokyo 104-0045 Japan Fax 81 3 3541 2685 Tel 81 3 3542 2511 ext 4452 E-mail tochiya@ These authors contributed equally to this work Received 30 October 2007 revised 26 December 2007 accepted 10 January 2008 doi The specific features of the plasticity of adult stem cells are largely unknown. Recently we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells AT-MSCs . To identify the genes responsible for hepatic differentiation we examined the gene expression profiles of AT-MSC-derived hepatocytes AT-MSC-Hepa using several microarray methods. The resulting sets of differentially expressed genes 1639 clones were comprehensively analyzed to identify the pathways expressed in AT-MSC-Hepa. Clustering analysis revealed a striking similarity of gene clusters between AT-MSC-Hepa and the whole liver indicating that AT-MSC-Hepa were similar to liver with regard to gene expression. Further analysis showed that enriched categories of genes and signaling pathways such as complementary activation and the blood clotting cascade

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