TAILIEUCHUNG - Genetic Engineering Basics New Applications and Responsibilities Part 8

Tham khảo tài liệu 'genetic engineering basics new applications and responsibilities part 8', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 130 Genetic Engineering - Basics New Applications and Responsibilities Mundembe et al. 2009 . In an experiment to evaluate the mechanisms of pathogen-derived resistance N. benthamiana was transformed with recombinant pBI 121 carrying various forms of the CABMV coat protein gene following the method of co-cultivation of leaf explants with A. tumefaciens described by An et al. 1987 . The constructs used were pBI121-CPk which results in an expressed CABMV coat protein pBI121-PC which results in antisense CP pBI121-CPstop which results in a form of the CP mRNA that cannot be translated and CPcore which results in only the core region of the CP together with a pBI121 control. Evaluation of the responses of transgenic plants obtained indicate that coat protein-mediated resistance only results in delayed symptom development while RNA mediated approaches may result in recovery or immunity. Out of 68 CP expressing transgenic plants challenged with CABMV 19 expressed delayed symptom development and none displayed immunity. Out of 26 CP stop lines 3 displayed delayed symptom development 4 tolerance and 3 recovery phonotypes. Out of 49 antisense lines 1 displayed delayed symptom delayed symptom development and 3 lines showed modified symptoms. At the time of carrying out these experiments cowpea could not be transformed in a reliable reproducible manner and many research groups were working towards developing a suitable transformation procedure. However the experiments with transgenic tobacco served the purpose of evaluating the effectiveness of the different approaches. Coat protein mediated resistance would only result in delayed symptom development RNA mediated approaches are likely to give higher levels of resistance maybe even immunity. Therefore as the method for cowpea transformation become available one would know which particular constructs to use to get the desired levels of resistance. 4. Microprojectile bombardment biolistics Microprojectile bombardment also known

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