TAILIEUCHUNG - Báo cáo khoa học: "Changes of biomarkers with oral exposure to benzo(a)pyrene, phenanthrene and pyrene in rats"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Changes of biomarkers with oral exposure to benzo(a)pyrene, phenanthrene and pyrene in rats | J. Vet. Sci. 2007 8 4 361-368 JOURNAL OF Veterinary Science Changes of biomarkers with oral exposure to benzo a pyrene phenanthrene and pyrene in rats Hwan Goo Kang1 Sang Hee Jeong1 Myung Haing Cho2 Joon Hyoung Cho1 National Veterinary Research and Quarantine Service Anyang 430-824 Korea 2College of Veterinary Medicine Seoul National University Seoul 151-742 Korea Polycyclic aromatic hydrocarbons PAHs are ubiquitous environmental contaminants present in air and food. Among PAHs benzo a pyrene BaP phenanthrene PH and pyrene PY are considered to be important for their toxicity or abundance. To investigate the changes of biomarkers after PAH exposure rats were treated with BaP 150 gg kg alone or with PH 4 300 gg kg and PY 2 700 gg kg BPP group by oral gavage once per day for 30 days. 7-ethoxyresorufin-O-deethylase activity in liver microsomal fraction was increased in only BaP groups. The highest concentration ng g of BaP was found in muscle of rats treated with BaP alone at 20 days of treatment it was ng g in BPP treated rats at 30 days of treatment. The highest PH concentration was ng g in muscle and ng g in fat and for PY it was ng g in muscle and ng g in fat in BPP groups. In urine 114-161 ng ml 3-OH-PH was found while PH was 41-69 ng ml during treatment. 201-263 ng ml 1-OH-PY was found while PH was 9-17 ng ml in urine. The level of PY PH and their metabolites in urine was rapidly decreased after withdrawal of treatment. This study suggest that 1-OH-PY in urine is a sensitive biomarker for PAHs it was the most highly detected marker among the three PAHs and their metabolites evaluated during the exposure period and for 14 days after withdrawal. Key words benzo a pyrene biomarker PAHs phenanthrene pyrene Introduction Polycyclic aromatic hydrocarbons PAHs are by-products from incomplete combustion or pyrolysis of organic materials containing carbon and hydrogen and are present as mixtures in air and food 41 . Some of the PAHs are clas

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