TAILIEUCHUNG - Báo cáo khoa học: "Immunohistochemical study of osteopontin in boar testis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical study of osteopontin in boar testis | J. Vet. Set. 2007 8 2 107-110 JOURNAL OF Veterinary Science Immunohistochemical study of osteopontin in boar testis Seungjoon Kim Taekyun Shin Department of Veterinary Medicine and Applied Radiological Science Research Institute Cheju National University Jeju 690-756 Korea The expression of osteopontin OPN in boar testis was studied. Western blot analysis detected 66- and 32-kDa OPN immunopositive bands in the testes of adult boars. In postnatal piglets the 66-kDa OPN band was detected in the testes but not the 32-kDa band. In the newborn testis OPN immunostaining was seen in gonocytes and in some supporting cells in the seminiferous tubules as well as in interstitial Leydig cells. In the adult boar testis OPN immunoreactivity was detected in seminiferous tubules with varying intensities. Intense OPN immunostaining was seen in the residual bodies and acrosomes in the spermatids while occasionally OPN immunostaining was seen in spermatogonia and various stage of spermatocytes but in few Sertoli cells in the seminiferous tubules. In addition Leydig cells in adult boars were weakly immunostained with OPN. These findings suggest that OPN is detected in the majority of germ cells and is involved in spermatogenesis in boar testis. Key words boar osteopontin testis Introduction Osteopontin OPN is a highly acidic glycoprotein that was initially isolated from the mineralized matrix of bovine diaphyseal bone 2 8 . Subsequently it has been detected in several tissues including the kidney 16 brain 10 cancers 13 and body fluids including breast milk 7 and male seminal fluids 1 . Although the function of OPN depends on the type of cell in which it is expressed its major roles are thought to be in cell adhesion 13 and cell activation proliferation leading to the production of intracellular signals 14 In the male reproductive system OPN has been detected in a variety of male tissues including testis and epididymis as well as sperm 4 9 11 15 and it has been known to serve as one of

TÀI LIỆU LIÊN QUAN
TỪ KHÓA LIÊN QUAN
TÀI LIỆU MỚI ĐĂNG
TAILIEUCHUNG - Chia sẻ tài liệu không giới hạn
Địa chỉ : 444 Hoang Hoa Tham, Hanoi, Viet Nam
Website : tailieuchung.com
Email : tailieuchung20@gmail.com
Tailieuchung.com là thư viện tài liệu trực tuyến, nơi chia sẽ trao đổi hàng triệu tài liệu như luận văn đồ án, sách, giáo trình, đề thi.
Chúng tôi không chịu trách nhiệm liên quan đến các vấn đề bản quyền nội dung tài liệu được thành viên tự nguyện đăng tải lên, nếu phát hiện thấy tài liệu xấu hoặc tài liệu có bản quyền xin hãy email cho chúng tôi.
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.