TAILIEUCHUNG - Báo cáo khoa học: "Establishment of a canine spinal cord injury model induced by epidural balloon compression"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Establishment of a canine spinal cord injury model induced by epidural balloon compression | J. Vet. Set. 2007 8 1 89-94 JOURNAL OF Veterinary Science Establishment of a canine spinal cord injury model induced by epidural balloon compression Ji-Hey Lim1 Chang-Su Jung1 Ye-Eun Byeon1 Wan Hee Kim1 Jung-Hee Yoon3 Kyung-Sun Kang2 Oh-kyeong Kweon1 1 Department of Veterinary Surgery 2Laboratory of Stem Cell and Tumor Biology Department of Veterinary Public Health 3Department of Veterinary Radiology College of Veterinary Medicine Seoul National University Seoul 151-742 Korea A model that provides reproducible submaximal yet sufficient spinal cord injury is needed to allow experiments leading to development of therapeutic techniques and prediction of clinical outcome to be conducted. This study describes an experimental model for spinal cord injury that uses three different volumes of balloon inflation and durations of compression to create a controlled gradation outcome in adult dogs. Twenty-seven mongrel dogs were used for this study. A 3-french embolectomy catheter was inserted into the epidural space through a left hemilaminectomy hole at the L4 vertebral arch. Balloons were then inflated with 50 100 or 150 pl of a contrast agent at the LI level for 6 12 or 24 h and spinal canal occlusion SCO measured using computed tomography. Olby score was used to evaluate the extent of spinal cord injury and a histopathologic examination was conducted 1 week after surgery. The SCO of the 50 100 and 150 pl inflations was 22-46 51-70 and 75-89 respectively p . Olby scores were diminished significantly by a combination of the level of SCO and duration of inflation in all groups. Olby scores in the groups of 150 pl-12 h 150 pl-24 h and 100 pl-24 h were 0 and respectively. Based on these results a SCO 50 for 24 h and 75 for 12 h induces paraplegia up to a week after spinal cord injury. Key words dog balloon catheter spinal cord compression injury spinal cord occlusion Introduction Spinal cord injury models have been developed for studies related to development of .

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