TAILIEUCHUNG - Báo cáo sinh học: "Spontaneous sister chromatid exchanges in mitotic chromosomes of cattle"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Spontaneous sister chromatid exchanges in mitotic chromosomes of cattle | 385 Genet Sei Evol 1995 27 385-393 Elsevier INRA Original article Spontaneous sister chromatid exchanges in mitotic chromosomes of cattle Bos taurus L D Di Berardino1 MB Lioi2 MR Scarfi3 V Jovino1 p Marigliano1 1 University of Naples Federico II Department of Animal Science 80055 Portici Naples 2 University of Basilicata Department of Animal Science 85100 Potenza 3CNR-IRECE 80124 Naples Italy Received 9 September 1994 accepted 12 May 1995 Summary - Peripheral blood lymphocytes from 4 cattle donors were grown for 2 cell cycles in the presence of and Hg ml of 5-bromodeoxyuridine in order to detect the spontaneous level of sister chromatid exchanges and the dose-response relationships at low bromodeoxyuridine levels. Sister chromatid exchanges were counted on 50 s cycle metaphase spreads randomly scored for each donor at each dose. The mean rate of sister chromatid exchanges cell at pg ml of BUdR was which can be considered as spontaneous. The dose-response curve increased more rapidly from to pg ml of bromodeoxyuridine and less rapidly at higher concentrations. The frequency distribution of sister chromatid exchanges cell and that of chromosomes showing various numbers of exchanges followed the Poisson probability function only within the range of to pg ml of bromodeoxyuridine. The usefulness of determining the spontaneous level of sister chromatid exchanges in domestic animals is discussed in relation to its possible application for selection programs. spontaneous sister chromatid exchange SCE lymphocyte culture cattle Resume Estimation des échanges interchromatidiques spontanés dans les chromosomes mitotiques de bovin Bos taurus L . Des cultures de lymphocytes obtenus à partir du sang périphérique de 4 donneurs bovins ont été réalisées pendant 2 cycles cellulaires. Ces cultures sont effectuees en presence de 0 10 0 25 0 50 1 0 2 5 et 5 0 pg ml de bromodéoxyuridine BrdU . Nous avons étudié les relations réponse

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