TAILIEUCHUNG - Báo cáo khoa học: "Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan | J. Vet. Set. 2006 7 1 43-46 JOURNAL OF Veterinary Science Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan Muhammad Wasif Malik Najma Ayub Irfan Zia Qureshi Department of Biological Sciences Quaid-i-Azam University Islamabad . Box 45320 Pakistan Infectious bursal disease IBD is an acute and highly contagious disease of young chickens caused by Birnavứus. Mortality of infected birds can be best prevented ff injected with antibodies. The present study was an attempt to raise specific hyper-immune polyclonal antibodies against IBD virus in Pakistan. Commercial layers divided into four groups were injected with IBD vaccine subcutaneously according to four different treatment regimens. Eggs were collected daily and antibodies were purified from yolk with dextran sulphate. Titers of antibodies in serum and yolk were evaluated with enzyme linked immunosorbant assay and agar gel precipitation test. Antibody titers were significantly higher in yolk than serum. Eggs collected at 28 days post-vaccination had maximum antibody titers. Of treatment regimens Tj was found to be most effective for hyperimmunization. Lyophilized antibodies stored at 4 c did not lose their activity till the end of experiment. IBD virus infected birds were injected with purified antibodies which induced 92 recovery as compared to control birds. The study implicates that the purified antibodies may be useful as a therapeutic agent to cure IBD infected birds. Key words immunoglobulin Y immunoglobulin therapy passive antibody transfer passive immunotherapy Introduction Infectious bursal disease IBD is an acute highly contagious viral disease of growing chickens with worldwide occurrence. IBĐ was observed in chickens in 1957 in Gumboro district of Delaware USA. Bfrds that survive the disease are permanently immunosuppressed therefore more susceptible to other disease causing agents and do not respond adequately to vaccinations. The bursa of Fabricius BF

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