TAILIEUCHUNG - Báo cáo khoa hoc:" Construction and characterization of a bovine BAC library with four genome-equivalent coverage"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Construction and characterization of a bovine BAC library with four genome-equivalent coverage | Genet Sei. Evol. 33 2001 543-548 INRA EDP Sciences 2001 543 Original article Construction and characterization of a bovine BAC library with four genome-equivalent coverage André Eggena Mathieu Gautiera Alain Billautd Elisabeth Petita Hélène Hayes a Pascal Laurenta Catherine Urbanb Martha Pfister-Genskowc Ken Eilertsenc Michael D. Bishopc a Laboratoire de génétique biochimique et de cytogénétique Departement de génétique animale Institut national de la recherche agronomique 78352 Jouy-en-Josas Cedex France b Fondation Jean Dausset Centre d etude du polymorphisme humain 27 rue Juliette Dodu 75010 Paris France c Infigen Inc 1825 Infinity Drive DeForest WI 53532 USA d Molecular Engines Laboratories 20 rue Bouvier 75011 Paris France Received 21 March 2001 accepted 16 May 2001 Abstract - a bovine artificial chromosome BAC library of 105 984 clones has been constructed in the vector pBeloBACll and organized in 3-dimension pools and high density membranes for screening by PCR and hybridization. The average insert size determined after analysis of 388 clones was estimated at 120 kb corresponding to a four genome coverage. Given the fact that a male was used to construct the library the probability of finding any given autosomal and X or Y locus is respectively and . The library was screened for 164 microsatellite markers and an average of superpools was positive for each PCR system. None of the 50 or so BAC clones analysed by FISH was chimeric. This BAC library increases the international genome coverage for cattle to around 28 genome equivalents and extends the coverage of the ruminant genomes available at the Inra resource center to 15 genome equivalents. bovine BAC library mapping 1. introduction In cattle numerous reports have identified genomic regions corresponding to economically important traits 3 5 7 based on low to medium density marker maps. The density of these maps however is not sufficient to identify DNA Correspondence and reprints E-mail .

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