TAILIEUCHUNG - Báo cáo khoa học: "An ultrastructural study on cytotoxic effects of mono(2-ethylhexyl) phthalate (MEHP) on testes in Shiba goat in vitro"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: An ultrastructural study on cytotoxic effects of mono(2-ethylhexyl) phthalate (MEHP) on testes in Shiba goat in vitro | J. Vet. Sci. 2004 5 3 235-240 JOURNAL OF Veterinary Science An ultrastructural study on cytotoxic effects of mono 2-ethylhexyl phthalate MEHP on testes in Shiba goat in vitro Bibin Bintang Andriana1 3 Tat Wei Tay1 Ishii Maki1 Mohammad Abdul Awal1 Yoshiakira Kanai1 Masamichi Kurohmaru1 Yoshihiro Hayashi2 Department of Veterinary Anatomy Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo 113-865 Japan Department of Global Animal Resource Science Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo 113-865 Japan Faculty of Animal Husbandry Bogor Agriculture University Kampus Darmaga Bogor 16680 Indonesia In this study the effects of mono 2-ethylhexyl phthalate MEHP one of metabolites of di 2-ethylhexyl phthalate on immature Shiba goat testes in vitro were examined. The testes of 2-month-old Shiba goats were cut into smaller pieces and seeded in medium. At 1 3 6 and 9 hr after administration of MEHP at various concentrations 0 100 nmol ml-1 1 nmol ml-1 and 1 X 10-3 nmol ml-1 respectively the specimens were obtained for light and transmission electron microscopic observations. As a result at 1 hr after exposure to MEHP the vacuolization and nuclear membrane rupture appeared in Sertoli cells. Such alterations tended to gradually increase in number in time-and dose-dependent manners. Moreover by MEHP treatment apoptotic spermatogenic cells characterized with chromatin condensation cytoplasm shrinkage without membrane rupture still functioning cell organelles and packed cell contents in membrane-bounded bodies apoptotic Sertoli cells characterized with nuclear membrane lysis nuclear condensation necrotic spermatogenic cells characterized with swollen and ruptured mitochondria plasma membrane lysis spilt cell contents and chromatin clumps and necrotic Sertoli cells characterized with marginated chromatins along the nuclear membrane ruptured vesicles within the MNB some swollen and ruptured cell organelles . .

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