TAILIEUCHUNG - Báo cáo khoa hoc:"The 5S rDNA of the bivalve Cerastoderma edule: nucleotide sequence of the repeat unit and chromosomal location relative to 18S-28S rDNA"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: The 5S rDNA of the bivalve Cerastoderma edule: nucleotide sequence of the repeat unit and chromosomal location relative to 18S-28S rDNA | Genet. Sei. Evol. 31 1999 509-518 Inra Elsevier Paris 509 Original article The 5S rDNA of the bivalve Cerastoderma edule nucleotide sequence of the repeat unit and chromosomal location relative to 18S-28S rDNA Ana Insua Ruth Freire Josefina Mendez Departamento de Biologia Celular y Molecular Universidade da Coruna A Zapateira s n 15071 A Coruna Spain Received 12 February 1999 accepted 2 July 1999 Abstract - The whole 5S rDNA repeated unit of the bivalve Cerastoderma edule was amplified by PCR and several clones were sequenced. In addition the PCR product from several individuals was digested with restriction enzymes. The results obtained indicate that 5S rDNA is organized in tandem repeats of 544-546 bp 120 of which could represent the coding region and 424-426 the spacer region. Minimal intra- and inter-individual variation was detected always within the spacer region. In comparison to the published 5S rRNA sequences of three other bivalves c. edule displays a maximum of four different nucleotide positions. A specific probe of c. edule 5S rDNA was generated by PCR and used for FISH. Five chromosome pairs were identified that carried a cluster of 5S rDNA at the telomere of the long arm. After performing FISH with a heterologous 18S-28S rDNA probe and C-banding absence of linkage between 5S and 18S-28S rDNA was demonstrated. Inra Elsevier Paris 5S rRNA gene non-transcribed spacer Cerastoderma edule I FISH Resume L ADNr 5S chez le bivalve Cerastoderma edule sequence nucléotidique de 1 unité de repetition et localisation chromosomique par rapport à 1 ADNr 18S-28S. L unite de repetition complete de 1 ADNr 5S a été amplifiee par PCR chez le bivalve Cerastoderma edule et plusieurs clones ont été sequences. En outre le produit de PCR de plusieurs individus a été digếré par des enzymes de restriction. Les résultats obtenus indiquent que 1 ADNr 5S est organise sous forme de repetitions en tandem dont 1 unité mesure 544-546 pb parmi lesquelles 120 pourraient representer la .

• Báo cáo khoa học
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học