TAILIEUCHUNG - Báo cáo khoa học: "Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows | J. Vet. Sci. 2004 5 1 29-39 JOURNAL OF Veterinary Science Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows Yong Ho Park Yi Seok Joo1 Joo Youn Park2 Jin San Moon1 So Hyun Kim Nam Hoon Kwon Jong Sam Ahn1 William C. Davis2 and Christopher J. Davies2 Department of Microbiology College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea 1 Department of Bacteriology and Parasitology National Veterinary Research and Quarantine Service Anyang 430-824 Korea 2Department of Veterinary Microbiology and Pathology College of Veterinary Medicine Washington State University Pullman WA 99164-7040 USA Bovine mastitis is an infectious disease with a major economic influence on the dairy industry worldwide. Many factors such as environment pathogen and host affect susceptibility or resistance of an individual cow to bovine mastitis. Recently there has been considerable interest in defining genetic and immunological markers that could be used to select for improved disease resistance. In this study we have analyzed the lymphocyte subpopulations of mastitis-resistant and susceptible cows using monoclonal antibodies specific for bovine leukocyte differentiation antigens and flow cytometry. We have also used a microarray typing technique to define the bovine leukocyte antigen BoLA class I and class II haplotypes associated with resistance or susceptibility to bovine mastitis. A striking finding of the present study is that susceptibility to mastitis was associated with major histocompatibility complex MHC haplotypes that have only a single set of DQ genes. The study also revealed that susceptible cows had CD4 CD8 ratios of less than one in both their mammary gland secretions and peripheral blood. These results raise the possibility that the number of DQ genes that a cow has and or a cow s CD4 CD8 ratio could be used as indicators of .

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