TAILIEUCHUNG - Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC | Virology Journal BioMed Central Short report Open Access Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC Moti L Chapagain Taylor Nguyen Thomas Bui Saguna Verma and Vivek R Nerurkar Address Retrovirology Research Laboratory Department of Tropical Medicine Medical Microbiology and Pharmacology Asia-Pacific Institute of Tropical Medicine and Infectious Diseases John A. Burns of School of Medicine University of Hawaii 651 Ilalo Street BSB 325AA Honolulu HaWaii 96813 USA Email Moti L Chapagain - moti@ Taylor Nguyen - minh@ Thomas Bui - tuanb@ Saguna Verma - saguna@ Vivek RNerurkar - nerurkar@ Corresponding author Published 09 January 2006 Received 04 September 2005 Accepted 09 January 2006 Virology Journal 2006 3 3 doi 1743-422X-3-3 This article is available from http content 3 1 3 2006 Chapagain et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Human polyomavirus JC JCV the etiological agent of the disease progressive multifocal leukoencephalopathy PML affects immunocompromised patients particularly patients with AIDS. In vitro studies of JCV infection are hampered by the lack of sensitive JCV quantitation tests. Although the hemagglutination HA assay has been routinely employed for in vitro quantitation of JCV its sensitivity is severely limited. We have employed a real-time PCR assay which compares favorably with the HA assay for the in vitro quantitation of JCV. JCV Madl propagated in primary human fetal glial PHFG cells in two independent laboratories was purified and quantitated by the HA assay. Both batches of purified JCV Mad1 were then serially diluted in Dulbecco s Modified

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