TAILIEUCHUNG - Báo cáo khoa học: Complete high-density lipoproteins in nanoparticle corona

In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, tri-glycerides and phospholipids from human plasma, and that the binding reaches saturation. . | ễFEBS Journal Complete high-density lipoproteins in nanoparticle corona Erik Hellstrand1 Iseult Lynch2 Astra Andersson3 Torbjorn Drakenberg1 Bjorn Dahlback3 Kenneth A. Dawson2 Sara Linse1 2 and Tommy Cedervall1 2 1 BiophysicalChemistry Lund University Sweden 2 Schoolof Chemistry and ChemicalBiology University College Dublin Ireland 3 ClinicalChemistry University Hospital Malmo Lund University Sweden Keywords apolipoprotein HDL lipids nanotoxicology transport pathways Correspondence K. A. Dawson Schoolof Chemistry and ChemicalBiology University College Dublin Belfield Dublin 4 Ireland Fax 353 1 716 2415 Tel 353 1 716 2447 E-mail kenneth@ T. Cedervall Department of Biophysical Chemistry Lund University Box 124 SE-22100 Lund Sweden Fax 46 46 222 4116 Tel 46 46 222 8240 E-mail tcedervall@ Received 4 March 2009 revised 8 April 2009 accepted 15 April 2009 In a biological environment nanoparticles immediately become covered by an evolving corona of biomolecules which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here for the first time we show using size exclusion chromatography NMR and pull-down experiments that copolymer nanoparticles bind cholesterol triglycerides and phospholipids from human plasma and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein HDL . By using fractionated lipoproteins we show that HDL binds to copolymer nanoparticles with much higher specificity than other lipoproteins probably mediated by apolipoprotein A-I. Together with the previously identified protein binding patterns in the corona our results imply that copolymer nanoparticles bind complete HDL complexes and may be recognized by living systems as HDL complexes opening up these transport pathways to nanoparticles. Apolipoproteins have been .

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