TAILIEUCHUNG - Báo cáo khoa học: "Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in Large Intestines of C57BL/6 Mice"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in Large Intestines of C57BL/6 Mice | J. Vet. Sci. 2002 3 3 233-238 JOURNAL OF Veterinary Science Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in Large Intestines of C57BL 6 Mice Tae-Su Ham College of Natural Resources Yeungnam University Received June 5 2002 Accepted August 23 2002 Abstract The regional distributions and relative frequencies of some gastrointestinal endocrine cells in the three portions cecum colon and rectum of the large intestinal tract of C57BL 6 mice were examined with immunohistochemical method using 7 types of specific antisera against chromogranin A CGA serotonin somatostatin human pancreatic polypeptide HPP glucagon gastrin and cholecyctokinin CCK -8. In this study all 3 types of immunoreactive IR cells were identified. Most of these IR cells in the large intestinal portion were generally spherical or spindle in shape open-typed cell while cells with a round shape close-typed cell were found in the intestinal gland. Their relative frequencies varied according to each portion of the large intestinal tract. CGA-IR cells were found throughout the whole large intestinal tract but were most predominant in the colon. Serotonin-IR cells were detected throughout the whole large intestinal tract and showed highest frequency in the colon. Peculiarly glucagon-IR cells were restricted to the colon with a low frequency. However no somatostatin- HPP- gastrin- and CCK-8-IR cells were found in the large intestinal tract. In conclusion some peculiar distributional patterns of large intestinal endocrine cells were identified in C57BL 6 mice. Key words gastrointestinal endocrine cell immunohistochemistry C57BL 6 mouse Introduction C57BL 6 mice are inbred black mice and are probably the most widely used of all the inbred strains. Although in many ways they appear to be atypical of inbred strains of Corresponding author College of Natural Resources Yeungnam University Dae-dong 214-1 Kyungsan 712-749 Republic of Korea. Tel 053-810-2992 E-mail shtae@ .

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