TAILIEUCHUNG - Báo cáo khoa học: Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions – contribution and expression from motor neurons

The expression and localization of the proline-rich membrane anchor (PRiMA), an anchoring protein of tetrameric globular form acetylcholines-terase (G4 AChE), were studied at vertebrate neuromuscular junctions. Both muscle and motor neuron contributed to this synaptic expression pattern. During the development of rat muscles, the expression of PRiMA and AChET and the enzymatic activity increased dramatically; however, the proportion of G4AChE decreased. | ỊFEBS Journal Restricted localization of proline-rich membrane anchor PRiMA of globular form acetylcholinesterase at the neuromuscular junctions - contribution and expression from motor neurons K. Wing Leung Heidi Q. Xie Vicky P. Chen Mokka K. W. Mok Glanice K. Y. Chu Roy C. Y. Choi and Karl W. K. Tsim Department of Biology and Center for Chinese Medicine The Hong Kong University of Science and Technology China Keywords acetylcholinesterase molecular form muscle fiber type neuromuscular junction proline-rich membrane anchor Correspondence K. W. K. Tsim Department of Biology The Hong Kong University of Science and Technology Clear Water Bay Road Kowloon Hong Kong SAR China Fax 852 2358 1559 Tel 852 2358 7332 E-mail BOTSIM@ Received 21 November 2008 revised 11 March 2009 accepted 25 March 2009 doi The expression and localization of the proline-rich membrane anchor PRiMA an anchoring protein of tetrameric globular form acetylcholinesterase G4 AChE were studied at vertebrate neuromuscular junctions. Both muscle and motor neuron contributed to this synaptic expression pattern. During the development of rat muscles the expression of PRiMA and AChET and the enzymatic activity increased dramatically however the proportion of G4 AChE decreased. G4 AChE in muscle was recognized specifically by a PRiMA antibody indicating the association of this enzyme with PRiMA. Using western blot and ELISA both PRiMA protein and PRiMA-linked G4 AChE were found to be present in large amounts in fast-twitch muscle . tibialis but in relatively low abundance in slow-twitch muscle . soleus . These results indicate that the expression level of PRiMA-linked G4 AChE depends on muscle fiber type. In parallel the expression of PRiMA AChET and G4 AChE also increased in the spinal cord during development. Such expression in motor neurons contributed to the synaptic localization of G4 AChE. After denervation the expression of PRiMA AChET and G4 AChE .

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