TAILIEUCHUNG - Báo cáo y học: "Do we use the appropriate controls for the identification of informative methylation markers for early cancer detection"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: IDo we use the appropriate controls for the identification of informative methylation markers for early cancer detection? | Correspondence Do we use the appropriate controls for the identification of informative methylation markers for early cancer detection Yasser Riazalhosseini and Jorg D Hoheisel Address Functional Genome Analysis Deutsches Krebsforschungszentrum Im Neuenheimer Feld 580 69120 Heidelberg Germany. Correspondence Yasser Riazalhosseini. Email Published 26 November 2008 Genome Biology 2008 9 405 doi gb-2008-9-11-405 The electronic version of this article is the complete one and can be found online at http 2008 9 11 405 2008 BioMed Central Ltd Abstract It is possible to miss potential DNA methylation markers of tumorigenesis because of the initial filtering of profiling results on the basis of inappropriate controls. Epigenetic programming - the variation of the chemical modifications of both histones and DNA - dictates the interpretation of the genetic code. For example different cell types can be distinguished by their at least partly distinct epigenetic status. Similarly tumor cells exhibit epigenetic patterns that vary from those of normal cells. These differences in epigenetic programming cause concomitant differences in gene-expression patterns. DNA methylation is the best-studied epigenetic marker. In the human genome DNA methylation occurs at cytosines that are located 5 to guanines known as CpG dinucleotides. Abnormal changes in methylation patterns found in human cancers include global demethylation of DNA with simultaneous hypermethylation of CpG-rich regions called CpG islands. These islands occur in promoter regions of many tumor suppressor genes and are usually not methylated in normal cells 1 2 . Because aberrant DNA-methylation events are both stable and abundant in tumors and also occur in the early stages of tumori-genesis detection of hypermethylated DNA is considered a most promising tool for the diagnosis of cancer. Specialized microarrays mass spectrometry and - more recently - ultrahigh-through-put .

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