TAILIEUCHUNG - Báo cáo sinh học: " HCV entry receptors as potential targets for siRNA-based inhibition of HCV"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: HCV entry receptors as potential targets for siRNA-based inhibition of HCV | Jahan et al. Genetic Vaccines and Therapy 2011 9 15 http content 9 1 15 GENETIC VACCINES AND THERAPY RESEARCH Open Access HCV entry receptors as potential targets for siRNA-based inhibition of HCV Shah Jahan Baila Samreen Saba Khaliq Bushra Ijaz Mahwish Khan Muhammad Hassan Siddique Waqar Ahmad and Sajida Hassan Abstract Background Hepatitis C virus HCV is a major health concern with almost 3 of the world s population 350 million individuals and 10 of the Pakistani population chronically infected with this viral pathogen. The current therapy of interferon-a and ribavirin against HCV has limited efficiency so alternative options are desperately needed. RNA interference RNAi which results in a sequence-specific degradation of HCV RNA has potential as a powerful alternative molecular therapeutic approach. Concerning viral entry the HCV structural gene E2 is mainly involved in virus attachment to the host cell surface receptors . CD81 tetraspanin scavenger receptor class B type 1 SR-B1 low density lipoprotein receptor LDLR and claudinJ CLDN1 . Results In this report we studied the relationship of the HCV receptors CD81 LDL CLDN1 and SR-B1to HCV infection. The potential of siRNAs to inhibit HCV-3a replication in serum-infected Huh-7 cells was demonstrated by treatment with siRNAs against HCV receptors which resulted in a significant decrease in HCV viral copy number. Conclusions Our data clearly demonstrate that the RNAi-mediated silencing of HCV receptors is among the first of its type for the development of an effective siRNA-based therapeutic option against HCV-3a. These findings will shed further light on the possible role of receptors in inhibition of HCV-3a viral titre through siRNA mediated silencing. Introduction HCV infection is a major health problem more than 350 million people worldwide and 10 of the population in Pakistan are chronically infected with this disease 1 2 . In 40-60 of HCV-infected individuals chronic infection is mainly

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