TAILIEUCHUNG - Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice | Wang et al. Genetic Vaccines and Therapy 2010 8 2 http content 8 1 2 GENETIC VACCINES AND THERAPY RESEARCH Open Access Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice Yaoli Wang1 2 3 Chunxue Bai2 Guansong Wang1 Diane Wang Xiaoming Cheng1 Jian Huang Dongpo Jiang Guisheng Qian1 and Xiangdong Wang 2 Abstract Background Allergen-induced imbalance of specific T regulatory Treg cells and T helper 2 cells plays a decisive role in the development of immune response against allergens. Objective To evaluate effects and potential mechanisms of DNA vaccine containing ovalbumin OVA and Fc fusion on allergic airway inflammation. Methods Bronchoalveolar lavage BAL levels of inflammatory mediators and leukocyte infiltration expression of CD11c CD80 and CD11c CD86 co-stimulatory molecules in spleen dendritic cells DCs circulating CD4 and CD8 T cells Foxp3 in spleen CD4 T cells and spleen CD4 T cells were measured in OVA-sensitized and challenged animals pretreated with pcDNA OVA-pcDNA Fc-pcDNA and OVA-Fc-pcDNA. Results OVA-Sensitized and challenged mice developed airway inflammation and Th2 responses and decreased the proliferation of peripheral CD4 and CD8 T cells and the number of spleen Foxp3 Treg. Those changes with increased INF-y production and reduced OVA-specific IgE production were protected by the pretreatment with OVA-Fc-pcDNA. Conclusion DNA vaccine encoding both Fc and OVA showed more effective than DNA vaccine encoding Fc or OVA alone through the balance of DCs and Treg. Introduction Allergic asthma is a Th2 lymphocyte-associated inflammatory airway disease characterized by airway eosinophilia goblet-cell hyperplasia variable airway obstruction and hyper-responsiveness 1 . The balance between allergen-specific T regulatory Treg cells and T helper 2 cells appears to be decisive in the development of the immune response against allergens 2 . .

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