TAILIEUCHUNG - Báo cáo sinh học: " A new plasmid vector for DNA delivery using lactococci"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: A new plasmid vector for DNA delivery using lactococci | Genetic Vaccines and Therapy BioMed Central Research A new plasmid vector for DNA delivery using lactococci Valeria Guimarães1 Sylvia Innocentin2 Jean-Marc Chatel3 Francois Lefevre4 Philippe Langella2 Vasco Azevedo1 and Anderson Miyoshi 1 Open Access Address 1Instituto de Ciências Biológicas Universidade Federal de Minas Gerais ICB-UFMG Belo Horizonte - MG Brasil 2INRA UR910 Unité d Ecologie et Physiologie du Système Digestif Domaine de Vilvert 78352 Jouy-en-Josas France 3INRA UR496 Unité d lmmuno-Allergie Alimentaire Domaine de Vilvert 78352 Jouy-en-Josas France and 4INRA UR892 Unité de Virologie et Immunologie Moléculaires Domaine de Vilvert 78352 Jouy-en-Josas France Email Valeria Guimarães - Sylvia Innocentin - Jean- Marc Chatel - jmchatel@ Francois Lefevre - Philippe Langella - Vasco Azevedo - vasco@ Anderson Miyoshi - miyoshi@ Corresponding author Published 10 February 2009 Received 15 October 2008 . J. . Accepted 10 February 2009 Genetic Vaccines and Therapy 2009 7 4 doi l479-0556-7-4 This article is available from http content 7 1 4 2009 Guimarães et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The use of food-grade lactococci as bacterial carriers to DNA delivery into epithelial cells is a new strategy to develop live oral DNA vaccine. Our goal was to develop a new plasmid named pValac for antigen delivery for use in lactococci. The pValac plasmid was constructed by the fusion of i a eukaryotic region allowing the cloning of an antigen of interest under the control of the pCMV eukaryotic promoter to be expressed .

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