TAILIEUCHUNG - Báo cáo sinh học: " Genome size in Calomys laucha and Calomys musculinus (Rodentia, Cricetidae)"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Genome size in Calomys laucha and Calomys musculinus (Rodentia, Cricetidae) | Genet Sei Evol 1993 25 109-120 @ Elsevier INRA . 8 JOIN W5W 109 Original article Genome size in Calomys laucha and Calomys musculinus Rodentia Cricetidae MA Ciccioli L Poggio Facultad de Ciencias Exactas y Naturales Departamento de Ciencias Biológicas Centro de Investigaciones Genéticas UNLP-CONICET-CIC CC4 Llavallol 1824 Buenos Aires Argentina Received 12 April 1991 accepted 28 December 1992 Summary - The DNA content of 2 related species Calomys laucha Thomas c 1 2n 64 fundamental number 74 and Calomys musculinus Fisher C m 2n 38 fundamental number 62 was studied using Feulgen microdensitometry using Mus domesticus as a control. Amounts of haploid DNA in the 2 species were significantly different Cl pg Cm pg p . The results were analyzed in relation to the total diploid karyotype length measured from synaptonemal complexes with a light microscope Cl pm Cm pm p and from mitotic metaphase chromosomes Cl rm Cm rm p the metacentric-submetacentric autosome number Cl 8 Cm 22 and the area of chromocenters showing positive staining with C-banding technique heterochromatin Cl 100 Cm . The DNA amount in pg per unit length of karyotype measured from synaptonemal complexes is higher in Calomys musculinus pg zm than in Calomys laucha pg zm . This indicates that there is not a constant amount of DNA associated with a given length of karyotype which suggests that the difference between the 2 species may involve differential packing of DNA. This could be due to genic differences differential interactions between genes and the cellular environment and or alteration of gene expression following the formation of new linkage groups due to chromosomal rearrangements. Calomys musculinus I Calomys laucha I C-value karyotype length synaptonemal complex Resume Taille du genome chez Calomys laucha et Calomys musculinus Rongeurs Cricétidés . La teneur en ADN de 2 espèces apparentées Calomys laucha Thomas Cl 2n 64

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