TAILIEUCHUNG - Báo cáo sinh học: "Biological imaging using secondary ions"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Biological imaging using secondary ions. | J. Biol. Journal of Biology BioMed Central Minireview Biological imaging using secondary ions Peter Williams Address Department of Chemistry and Biochemistry Arizona State University Tempe AZ 85287 USA. E-mail PW@ Published 5 October 2006 Journal of Biology 2006 5 18 The electronic version of this article is the complete one and can be found online at http content 5 6 18 2006 BioMed Central Ltd Abstract Biological materials are morphologically and chemically complex. A quantitative imaging tool is now available that can produce chemical and even metabolic information from morphological features as small as a few nanometers. Biological materials are immensely complex structures whose chemical composition can vary both temporally and on an extremely fine spatial scale. Biologists operate with mental models of the abundance distribution movement and metabolism of molecular and ionic species within these complex structures. A chemical imaging tool is now available that can illuminate such models by visualizing and quantifying these species with a spatial resolution of around 30 nm and the ability to obtain chemical information from features as small as a few nanometers. This tool is a novel mass spectrometer that generates chemical images and derived isotope ratio images using ions ejected directly from the surface of a sample by a probing ion beam focused to a spot as small as around 30 nm. In this issue of Journal of Biology Lechene and co-workers 1 report impressive results from such an instrument utilized by a collaboration of biologists and instrument experts at the National Resource for Imaging Mass Spectrometry established by Lechene at Harvard Medical School and Brigham and Women s Hospital. They call their technique multi-isotope imaging mass spectrometry MIMS . The first imaging secondary-ion mass spectrometer was developed by Georges Slodzian in the late 1950s for his PhD thesis at the University of Paris 2 . This novel instrument utilized .

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