TAILIEUCHUNG - Báo cáo " Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng "

We investigated the effects on ginseng cell and ginsenoside production when macro element concentrations were manipulated in the culture media. Biomass growth was greatest in the medium supplemented with strength NH4NO3, whereas ginsenoside accumulation was highest ( mg/g DW). At levels of strength KNO3, cell growth was maximum, but strength of KNO3 led to the greatest ginsenoside content ( mg/g DW). | VNU Journal of Science Natural Sciences and Technology 24 2008 248-252 Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng Panax vietnamensis Ha et Grushv. Nguyen Trung Thanh1 Ha Tuan Anh1 Paek Kee Yoeup2 1 Department of Biology Vietnam National University Hanoi 334 Nguyen Trai Hanoi Vietnam 2Department of Horticulture Chungbuk National University 361-763 Cheongju South Korea Received 15 November 2007 Abstract. We investigated the effects on ginseng cell and ginsenoside production when macro element concentrations were manipulated in the culture media. Biomass growth was greatest in the medium supplemented with strength NH4NO3 whereas ginsenoside accumulation was highest mg g DW . At levels of strength KNO3 cell growth was maximum but strength of KNO3 led to the greatest ginsenoside content mg g DW . High concentrations of MgSO4 were most favorable for both cell growth and ginsenoside accumulation up to mg g DW . Cell growth and ginsenoside content also increased in proportion to the concentration of CaCl2 in the medium with the greatest accumulation of ginsenoside mg g DW occurring at a strength. Keywords macro element suspension culture conical flask Panax. 1. Introduction Vietnamese ginseng was found at highland of Central Vietnam in 1973 and was regarded as a new species as Panax vietnamensis Ha et Grushv. 1985 . This is the most southern distribution of Panax genus Araliaceae . It is a secret medicine of the Sedang ethnic group as a miraculous life-saving plant drug used for the treatment of many serious diseases and for enhancing body strength in long journeys in high mountains. In recent years plant cell culture technology has successfully applied to the production of many useful secondary metabolites including pharmaceuticals pigments and other fine chemicals 1 2 . Ginsenosides also have Corresponding author. Tel. 84-4-8582178. E-mail thanhntsh@ been derived

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