TAILIEUCHUNG - Báo cáo sinh học: "Genetic differentiation of European grayling (Thymallus thymallus) populations in Serbia, based on mitochondrial and nuclear DNA analyses"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học quốc tế đề tài: Genetic differentiation of European grayling (Thymallus thymallus) populations in Serbia, based on mitochondrial and nuclear DNA analyses | Marie et al. Genetics Selection Evolution 2011 43 2 http content 43 1 2 GSE Ge n et i cs Selection Evolution RESEARCH Open Access Genetic differentiation of European grayling Thymallus thymallus populations in Serbia based on mitochondrial and nuclear DNA analyses 1 2 1 1 Sasa Marie Andrej Razpet Vera Nikolic Predrag Simonovic Abstract Background The structure and diversity of grayling Thymallus thymallus populations have been well studied in most of its native habitat however the southernmost populations of the Balkan Peninsula remain largely unexplored. The purpose of this study was to assess the genetic diversity of Serbian grayling populations detect the impact of stocking and provide guidelines for conservation and management. Methods Eighty grayling individuals were collected from four rivers Ibar Lim Drina and Rzav . The mitochondrial DNA control region CR 595 bp of the 3 end and 74 bp of flanking tRNA and the ATP6 gene 630 bp fragment were sequenced for 20 individuals five from each locality . In addition all individuals were genotyped with 12 microsatellite loci. The diversity and structure of the populations as well as the recent and ancient population declines were studied using specialized software. Results We detected three new haplotypes in the mtDNA CR and four haplotypes in the ATP6 gene of which three had not been described before. Previously one CR haplotype and two ATP6 gene haplotypes had been identified as allochthonous originating from Slovenia. Reconstruction of phylogenetic relations placed the remaining two CR haplotypes from the River Danube drainage of Serbia into a new clade which is related to the previously described sister Slovenian clade. These two clades form a new Balkan clade. Microsatellite marker analysis showed that all four populations are genetically distinct from each other without any sign of intrapopulation structure although stocking of the most diverse population Drina River was confirmed by mtDNA .

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