TAILIEUCHUNG - Báo cáo sinh học: " Assignment of CPS1, OTC, CRYD2, ARG2 and ASS genes to the chicken RH map"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Assignment of CPS1, OTC, CRYD2, ARG2 and ASS genes to the chicken RH map | Genet. Sel. Evol. 36 2004 593-599 INRA EDP Sciences 2004 DOI gse 2004019 593 Note Assignment of CPS1 OTC CRYD2 ARG2 and ASS genes to the chicken RH map Takeshi SHIMOGIRIa Natalia BoSAKb Mireille Morissonc Shin OKAMOTOa Kotaro KAWABEd Yoshizane MAEDAa Alain Vignalc Hiroshi YASUEb a Faculty of Agriculture Kagoshima University Kagoshima Japan b Genome Research Department National Institute of Agrobiological Sciences Tsukuba Japan c Laboratoire de génétique cellulaire Institut national de la recherche agronomique Castanet-Tolosan France d Gene Research Center Kagoshima University Kagoshima Japan Received 30 October 2003 accepted 27 April 2004 Abstract - An attempt was made to assign five genes CPS1 OTC ASS CRYD2 and ARG2 to chicken chromosomes GGA by radiation-hybrid mapping. OTC was assigned to GGA1 ARG2 to GGA5 CPS1 to GGA7 and CRYD2 to GGA19. ASS was not however assigned to a specific chromosomal position. ornithine-urea cycle chicken radiation hybrid mapping chromosomal assignment 1. INTRODUCTION The ornithine-urea cycle is an enzyme system that functions in the biosynthesis of urea. This cycle consists of five enzymes carbamyl phosphate synthetase I CPS1 ornithine transcarbamylase OTC argininosuccinate lyase ASL argininosuccinate synthetase ASS and arginase ARG . The ornithine-urea cycle has been shown to be conserved in many organisms from bacteria including E. coli to mammalian species and is involved in key physiological functions 2 . All animal species on land except for birds and reptiles so far examined are found to use the cycle for nitrogen excretion. Birds and reptiles are uricotelic in terms of nitrogen excretion so that they apparently do not require the ornithine-urea cycle for nitrogen excretion. However the activities of OTC ASL ASS and ARG have been reported in the Corresponding author hyasue@ 594 T. Shimogiri et al. chicken kidney 15 . In the chicken ASL activity was found in the product of the d2-crystallin CRYD2 gene showing .

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