TAILIEUCHUNG - Báo cáo sinh học: " Identification of a differentially expressed gene, ACL, between Meishan · Large White and Large White · "

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học quốc tế đề tài: Identification of a differentially expressed gene, ACL, between Meishan · Large White and Large White · | Genet. Sel. Evol. 40 2008 625-637 INRA EDP Sciences 2008 DOI gse 2008024 Available online at Original article Identification of a differentially expressed gene ACL between Meishan X Large White and Large White X Meishan F1 hybrids and their parents Zhu-Qing Ren Yan Wang Yong-Jie Xu Lin-Jie Wang Ming-Gang Lei Bo Zuo Feng-E Li De-Quan Xu Rong Zheng Chang-Yan Deng Si-Wen Jiang Yuan-Zhua Xiong Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture Key Laboratory of Agriculture Animal Genetics Breeding and Reproduction of Ministry of Education College of Animal Science Huazhong Agricultural University Wuhan 430070 China Received 23 January 2008 accepted 26 June 2008 Abstract - ATP-citrate lyase ACL one of the lipogenic enzymes catalyses the formation of acetyl-coenzyme A CoA involved in the synthesis of fatty acid and cholesterol. In pig very little is known about the ACL gene. In this work the mRNA differential display technique was used to analyse the differences in gene expression between Meishan and Large White pigs and the F1 hybrids of both direct and reciprocal crosses. Our results show that among the differentially expressed genes ACL is up-regulated in the backfat of the F1 hybrids. After cloning and analysing the fulllength cDNA and the 870 bp 5 -flanking sequence of the porcine ACL gene a C T mutation at position 97 bp upstream of the transcription site was detected. Luciferase activity detection showed that this mutation changed the transcriptional activity. In F1 hybrids the heterozygous genotype CT was more frequent than the homozygous genotypes CC and TT. Real-time PCR analysis showed that in Meishan pigs ACL mRNA expression was more abundant in individuals with genotype CT than in those with genotype CC or TT or in Large White pigs. These results indicate that the C T mutation affects ACL mRNA expression probably via the activator protein 2. differential gene expression ATP-citrate lyase promoter mutation .

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