TAILIEUCHUNG - Báo cáo sinh học: "AFLP linkage map of the Japanese quail Coturnix japonica"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: AFLP linkage map of the Japanese quail Coturnix japonica | 559 Genet Sei. Evol. 35 2003 559-572 INRA EDP Sciences 2003 DOI gse 2003039 Original article AFLP linkage map of the Japanese quail Coturnix japonica Odile Roussota Katia Fevea Florence Plisson-Petita Frederique Pitela Jean-Michel Faureb Catherine Beaumontb Alain Vignala a Laboratoire de génétique cellulaire Institut national de la recherche agronomique 31326 Castanet-Tolosan France b Station de recherches avicoles Institut national de la recherche agronomique 37380 Nouzilly France Received 26 December 2002 accepted 7 April 2003 Abstract - The quail is a valuable farm and laboratory animal. Yet molecular information about this species remains scarce. We present here the first genetic linkage map of the Japanese quail. This comprehensive map is based solely on amplified fragment length polymorphism AFLP markers. These markers were developed and genotyped in an F2 progeny from a cross between two lines of quail differing in stress reactivity. A total of 432 polymorphic AFLP markers were detected with 24 Taql EeoRl primer combinations. On average 18 markers were produced per primer combination. Two hundred and fifty eight of the polymorphic markers were assigned to 39 autosomal linkage groups plus the zw sex chromosome linkage groups. The linkage groups range from 2 to 28 markers and from to cM. The AFLP map covers a total length of 1516 cM with an average genetic distance between two consecutive markers of cM. This AFLP map can be enriched with other marker types especially mapped chicken genes that will enable to link the maps of both species and make use of the powerful comparative mapping approach. This AFLP map of the Japanese quail already provides an efficient tool for quantitative trait loci QTL mapping. Japanese quail AFLP genetic map linkage groups chromosomes 1. introduction Japanese quail are appreciated for meat and eggs. It is also a valuable laboratory species because of its small body size rapid generation interval and high .

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