TAILIEUCHUNG - Báo cáo sinh học: "Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding | Genet. Sel. Evol. 34 2002 649-656 649 INRA EDP Sciences 2002 DOI gse 2002029 Note Localisation of aphidicolin-induced break points in Holstein-Friesian cattle Bos taurus using RBG-banding Viviana RODRIGUEZa Silvia LLAMBÍa Alicia POSTIGLIONIa Karina GUEVARAa Gonzalo RINCÓNa Gabriel FERNÁNDEZb Beatriz MERNIESb María Victoria Arrugac a Department of Cell and Molecular Biology Genetic Section Laboratory of Genetics Analysis in Domestic Animals Faculty of Veterinary UDELAR A. Lasplaces 1550 CP 11600 Montevideo Uruguay b Department of Animal Production Animal breeding Section Faculty of Veterinary UDELAR Uruguay c Laboratory of Cytogenetics and Molecular Genetics Faculty of Veterinary Zaragoza University Spain Received 21 November 2001 accepted 1st July 2002 Abstract - Fragile sites FS seem to play a role in genome instability and may be involved in karyotype evolution and chromosome aberrations. The majority of common fragile sites are induced by aphidicolin. Aphidicolin was used at two different concentrations and rM to study the occurrence of FS in the cattle karyotype. In this paper a map of aphidicolin induced break points and fragile sites in cattle chromosomes was constructed. The statistical analysis indicated that any band with three or more breaks was significantly damaged P . According to this result 30 of the 72 different break points observed were scored as fragile sites. The Pearson correlation test showed a positive association between chromosome length and the number of fragile sites r . On the contrary 21 FS were identified on negative R bands while 9 FS were located on positive R bands. cattle chromosome fragile sites aphidicolin 1. INTRODUCTION Fragile sites are non-random chromosomal breaks or gaps observed in cells under folate-deficient conditions or in cells grown in the presence of Correspondence and reprints E-mail sllambi@ alipos@ 650 V Rodriguez et al. certain mutagens carcinogens or .

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