TAILIEUCHUNG - Báo cáo " Study on PAH degrading bacteria isolated from dioxin contaminated soil in Vietnam "

Bacterial community in dioxin contaminated soil and mud samples from Danang airport was enumerated and studied for their potential degradation capability against three-ring compound carbazol. The cell number was determined on nutrient rich medium and on mineral medium supplemented with carbazol. It has been showed that number of cells presented in mud samples was significantly higher than in soil samples, in the range of 1 magnitude order. About 50% of the total cell number in each sample grown on the medium with carbazol. Analyzing 12 representative strains selectively isolated on the carbazol containing medium by using the ARDRA method with two endonucleases HaeIII and MspI showed that. | VNU Journal of Science Natural Sciences and Technology 26 2010 27-35 Study on PAH degrading bacteria isolated from dioxin contaminated soil in Vietnam Nguyen Hong Minh1 Nguyen Thi Hanh1 Duong Van Hop2 Dinh Thuy Hang2 1 Faculty of Biology College of Science VNU 334 Nguyen Trai Hanoi Vietnam 2Institute of Microbiology and Biotechnology VNU 144 Xuan Thuy Hanoi Vietnam Received 17 September 2009 Abstract. Bacterial community in dioxin contaminated soil and mud samples from Danang airport was enumerated and studied for their potential degradation capability against three-ring compound carbazol. The cell number was determined on nutrient rich medium and on mineral medium supplemented with carbazol. It has been showed that number of cells presented in mud samples was significantly higher than in soil samples in the range of 1 magnitude order. About 50 of the total cell number in each sample grown on the medium with carbazol. Analyzing 12 representative strains selectively isolated on the carbazol containing medium by using the ARDRA method with two endonucleases HaeIII and MspI showed that bacterial community at the studied areas was highly diversified. However non from these isolates could degrade carbazol significantly. Via enrichment and isolation steps using carbazol as the only energy and carbon sources two carbazol degrading isolates R03 and R05 were obtained. Phylogenetic analyses based on 16S rDNA sequences showed that these strains were most affiliated to Bacillus and Archromobacter species the most closely related species were Achromobacter xylosoxydans 99 homology and Bacillus subtilis 98 homology . These strains were therefore designated the names Bacillus sp. R03 and Achromobacter sp. R05. Keywords Dioxin contamination carbazol degrading bacteria 16S rDNA ARDRA. 1. Introduction Dioxin is a group of highly toxic chemicals that are hardly degraded under natural condition 1 . Due to the high toxicity of dioxins to living organisms model compounds such as .

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