TAILIEUCHUNG - Modern Advances in Chromatography (Advances in Biochemical Engineering Biotechnology

Chromatography is the separation method for biological molecules from the analytical to the preparative scale. Few of the recent advances in life science, whether in "proteomics" or in the industrial production of recombinant therapeutics, would have been possible without the help of chromatographic separations. Concomitantly, chromatography nowadays stands for much more than a batch column packed with porous particles | Preface Due to their versatility and resolution chromatographic separations of complex mixtures of biologicals are used for many purposes in academia and industry. If anything recent developments in the life sciences have increased the interest and need for chromatography be it for quality control proteomics or the downstream processing of the high value products of modern biotechnology. However the many challenges of present day chromatograppy andespeciaily of th th HPLC of biomacromolecules such as proteins are also present in the mind of any practitioner. In fact some of these latter were such hindrances that much research was necessary in order to overcome and circumvent them. This book introduces the reader to some of the recently proposed solutions. Capillary electrochromatography CEC for example the latest and most promising branch of analytical chromatography is still hindered from finding broader application by difficulties related to something as simple as the packing of a suitable column. The latest solutions for this but also the state of art of CEC in general are discussed in the chapter written by Frantisek Svec. The difficulty of combining speed resolution and capacity when using the classical porous bead type stationary phases has even been called the dilemma of protein chromatography . Much progress has been made in this area by the advent of monolithic and related continuous stationary phases. The complex nature of many of the samples to be analyzed and separated in biochromatography often requires the use of some highly specific affinity ligands. Since they can be raised in a specific manner to many bioproducts protein ligands such as antibodies have allowed some very selective solutions in the past. However they also are known to have some disadvantages including the immunogenicity toxicity of ligands contaminating the final products or the low stability of such ligands which prevents repeated usage of the expensive columns. This challenge may .

• Hoá học
• Biochemical Engineering Biotechnology
• Chromatography
• chemical analysis
• the overall chemical analysis
• chemical analysis of materials
• Biotechnology
• Biomedical Electronics
• Medical Equipment
• Biomimetics
• Transport Phenomena
• Reacting Systems