TAILIEUCHUNG - Báo cáo hóa học: " Fluorescence Quenching of Alpha-Fetoprotein by Gold Nanoparticles: Effect of Dielectric Shell on Non-Radiative Decay"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Fluorescence Quenching of Alpha-Fetoprotein by Gold Nanoparticles: Effect of Dielectric Shell on Non-Radiative Decay | Nanoscale Res Lett 2010 5 1496-1501 DOI s11671-010-9668-0 NANO EXPRESS Fluorescence Quenching of Alpha-Fetoprotein by Gold Nanoparticles Effect of Dielectric Shell on Non-Radiative Decay Jian Zhu Jian-jun Li A-qing Wang Yu Chen Jun-wu Zhao Received 13 April 2010 Accepted 7 June 2010 Published online 15 June 2010 The Author s 2010. This article is published with open access at Abstract Fluorescence quenching spectrometry was applied to study the interactions between gold colloidal nanoparticles and alpha-fetoprotein AFP . Experimental results show that the gold nanoparticles can quench the fluorescence emission of adsorbed AFP effectively. Furthermore the intensity of fluorescence emission peak decreases monotonously with the increasing gold nanoparticles content. A mechanism based on surface plasmon resonance-induced non-radiative decay was investigated to illuminate the effect of a dielectric shell on the fluorescence quenching ability of gold nanoparticles. The calculation results show that the increasing dielectric shell thickness may improve the monochromaticity of fluorescence quenching. However high energy transfer efficiency can be obtained within a wide wavelength band by coating a thinner dielectric shell. Keywords Fluorescence quenching Gold nanoparticles Alpha-fetoprotein AFP Non-radiative decay Dielectric shell Introduction Noble metal colloids such as gold and silver nanoparticles allow effective fluorescence quenching over a broad range of wavelengths which is to be used across a vast spectrum of applications such as energy transfer assays for the detection of proteins 1-4 . As we know sensitive J. Zhu J. Li . Wang Y. Chen J. Zhao The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi an Jiaotong University Xian Ning West Road 28 710049 Xi an People s Republic of China e-mail nanoptzhao@ 0 Springer analytical technology for quantification of protein

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