TAILIEUCHUNG - Báo cáo khoa học: The ycaC-related protein from the amphioxus Branchiostoma belcheri (BbycaCR) interacts with creatine kinase

TheycaC-related gene, ycaCR, is uncharacterized, and has no assigned function to date. Here we clearly showed that the ycaC-related gene from the amphioxus Branchiostoma belcheri, BbycaCR, coded for a novel mem-ber of the isochorismatase superfamily, which is mainly localized in the mitochondrial fraction. | ỊFEBS Journal The ycaC-related protein from the amphioxus Branchiostoma belcheri BbycaCR interacts with creatine kinase Shengjuan Jiang1 2 z Xuemei Sun1 and Shicui Zhang1 1 Department of Marine Biology Ocean University of China Qingdao China 2 College of Life Science Anhui Science and Technology University Fengyang China Keywords amphioxus Branchiostoma creatine kinase isochorismatase superfamily ycaC-related protein Correspondence S. Zhang Department of Marine Biology Ocean University of China Qingdao 266003 China Fax 86 532 82032787 Tel 86 532 82032787 E-mail sczhang@ These authors contributed equally to this work Received 29 May 2007 revised 10 July 2008 accepted 18 July 2008 The ycaC-related gene ycaCR is uncharacterized and has no assigned function to date. Here we clearly showed that the ycaC-related gene from the amphioxus Branchiostoma belcheri BbycaCR coded for a novel member of the isochorismatase superfamily which is mainly localized in the mitochondrial fraction. Both pull-down and reverse pull-down analyses revealed that BbycaCR was able to interact with creatine kinase an enzyme involved in energy transduction in addition to binding to native ycaCR forming a homopolymer. Surprisingly neither isochorismatase nicotinamidase nor N-carbamoylsarcosine amidohydrolase activity was detected for BbycaCR although it possessed the putative catalytic triad of Asp19 Arg Lys 84 and Cys118 that is found in ycaC proteins. Both tissue section in situ hybridization and immunohistochemistry showed that BbycaCR was ubiquitously expressed in amphioxus although at different expression levels suggesting that BbycaCR plays a conserved fundamental cellular role in amphioxus. It is proposed that BbycaCR may be indirectly involved in energy transduction. doi Isochorismatase catalyzes the conversion of isochoris-mate in the presence of H2O into 2 3-dihydro-2 3-di-hydroxybenzoate and pyruvate via hydrolysis of a vinyl ether an uncommon .

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