TAILIEUCHUNG - Báo cáo khoa học: Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis

Several isoforms of the cAMP-dependent protein kinase catalytic subunit (C-subunit) were separated from the posterior adductor muscle and the mantle tissues of the sea mussel Mytilus galloprovincialisby cation exchange chromatography, and identified by: (a) protein kinase activity; (b) antibody recognition; | ỊFEBS Journal Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis Jose R. Bardales1 Ulf Hellman2 and J. A. Villamarin1 1 Departamento de Bioquimica e Bioloxia Molecular Facultade de Veterinaria Universidade de Santiago de Compostela Lugo Spain 2 Ludwig Institute for Cancer Research Uppsala Sweden Keywords cAMP-dependent protein kinase catalytic subunit C-subunit isoforms MALDI-IOF IOF MS Mytilus Correspondence J. A. Villamarin Departamento de Bioquimica e Bioloxia Molecular Facultade de Veterinaria Universidade de Santiago de Compostela Campus de Lugo 27002 Lugo Spain Fax 34 82 252 195 Tel 34 82 285 900 E-mail Received 28 March 2008 revised 4 July 2008 accepted 10 July 2008 doi Several isoforms of the cAMP-dependent protein kinase catalytic subunit C-subunit were separated from the posterior adductor muscle and the mantle tissues of the sea mussel Mytilus galloprovincialis by cation exchange chromatography and identified by a protein kinase activity b antibody recognition and c peptide mass fingerprinting. Some of the isozymes seemed to be tissue-specific and all them were phosphorylated at serine and threonine residues and showed slight but significant differences in their apparent molecular mass values which ranged from to kDa. The results from the MS analysis suggest that at least some of the mussel C-subunit isoforms arise as a result of alternative splicing events. Furthermore several peptide sequences from mussel C-subunits determined by de novo sequencing showed a high degree of homology with the mammalian Ca-isoform and contained some structural motifs that are essential for catalytic function. On the other hand no significant differences were observed in the kinetic parameters of C-subunit isoforms determined by using synthetic peptides as substrate and inhibitor. However the C-subunit isoforms .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• breast cancer
• biological activities
• environmental medicine
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies