TAILIEUCHUNG - báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development | BMC Plant Biology BioMed Central Open Access Methodology article An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development Karen E Reid Niclas Olsson James Schlosser Fred Peng and Steven T Lund Address Faculty of Land and Food Systems University of British Columbia Vancouver Canada Email Karen E Reid - kereid@ Niclas Olsson - neolsson@ James Schlosser - jamesschlosser3@ Fred Peng - frepeng@ Steven T Lund - stlund@ Corresponding author Published 14 November 2006 Received 19 July 2006 ni . D _. AAAZ J .IA I mo - -1 Accepted 14 November 2006 BMC Plant Biology 2006 6 27 doi 1471-2229-6-27 This article is available from http 1471-2229 6 27 2006 Reid et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Accuracy in quantitative real-time RT-PCR is dependent on high quality RNA consistent cDNA synthesis and validated stable reference genes for data normalization. Reference genes used for normalization impact the results generated from expression studies and hence should be evaluated prior to use across samples and treatments. Few statistically validated reference genes have been reported in grapevine. Moreover success in isolating high quality RNA from grapevine tissues is typically limiting due to low pH and high polyphenolic and polysaccharide contents. Results We describe optimization of an RNA isolation procedure that compensates for the low pH found in grape berries and improves the ability of the RNA to precipitate. This .

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