TAILIEUCHUNG - Báo cáo khoa học: "HIV-1 CRF 02 AG polymerase genes in Southern Ghana are mosaics of different 02 AG strains and the protease gene cannot infer subtypes"

HIV-1 CRF 02 AG polymerase genes in Southern Ghana are mosaics of different 02 AG strains and the protease gene cannot infer subtypes | Virology Journal BioMed Central Research HIV-1 CRF 02 AG polymerase genes in Southern Ghana are mosaics of different 02 AG strains and the protease gene cannot infer subtypes Kwamena W Sagoe 1 Magda Dwidar2 Theophilus K Adiku1 and Max Q Arens2 Open Access Address 1Clinical Virology Laboratory Department of Microbiology University of Ghana Medical School PO Box 4236 Accra Ghana and 2Retrovirus Laboratory Department of Pediatrics Washington University Medical School St Louis Missouri 63110 USA Email Kwamena W Sagoe - kwcsagoe@ Magda Dwidar - Dwidar_M@ Theophilus K Adiku - tekadiku@ Max Q Arens - Arens@ Corresponding author Published 26 February 2009 Virology Journal 2009 6 27 doi 1743-422X-6-27 Received 23 December 2008 Accepted 26 February 2009 This article is available from http content 6 1 27 2009 Sagoe et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Little is known about the detailed phylogeny relationships of CRF 02_AG HIV-1 polymerase genes in Ghana. The use of the protease gene of HIV-1 for subtyping has shown conflicting results. Methods The partial polymerase gene sequences of 25 HIV-1 strains obtained with Viroseq reagents were aligned with reference subtypes and alignments trimmed to a 300 bp protease 661 bp and 1005 reverse transcriptase sequence alignments. Phylogenetic relationships of these alignments were determined with the Neighbour-Joining method using 1000 replicates and recombination patterns determined for the sequences with RIP in the HIV sequence database. Results Unlike the other alignments the protease gene had nodes with bootstrap values 100 for repeat control sequences. Majority of the CRF 02_AG .

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