TAILIEUCHUNG - Báo cáo khoa học: "Hepatitis A virus (HAV) packaging size limit"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Hepatitis A virus (HAV) packaging size limit | Virology Journal BioMed Central Open Access Hepatitis A virus HAV packaging size limit Krishnamurthy Konduru Siham M Nakamura and Gerardo G Kaplan Address Laboratory of Hepatitis and Related Emerging Agents Center for Biologics Evaluation and Research Food and Drug Administration 8800 Rockville Pike Bethesda Maryland 20892 USA Email Krishnamurthy Konduru - Siham M Nakamura - Gerardo G Kaplan - gk@ Corresponding author Published 18 November 2009 Virology Journal 2009 6 204 doi I743-422X-6-204 Received 17 September 2009 Accepted 18 NOvember 2009 This article is available from http content 6 1 204 2009 Konduru et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hepatitis A virus HAV an atypical Picornaviridae that causes acute hepatitis in humans grows poorly in cell culture and in general does not cause cytopathic effect. Foreign sequences have been inserted into different parts of the HAV genome. However the packaging size limit of HAV has not been determined. The purpose of the present study is to investigate the maximum size of additional sequences that the HAV genome can tolerate without loosing infectivity. Results In vitro T7 polymerase transcripts of HAV constructs containing a 456-nt fragment coding for a blasticidin Bsd resistance gene a 1 098-nt fragment coding for the same gene fused to GFP GFP-Bsd or a 1 032-nt fragment containing a hygromycin Hyg resistance gene cloned into the 2A-2B junction of the HAV genome were transfected into fetal Rhesus monkey kidney FRhK4 cells. After antibiotic selection cells transfected with the HAV construct containing the resistance gene for Bsd but not the .

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