TAILIEUCHUNG - Báo cáo khoa học: "Phylogenetic analysis of Newcastle disease viruses isolated from waterfowl in the Upper Midwest Region of the United States"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Phylogenetic analysis of Newcastle disease viruses isolated from waterfowl in the Upper Midwest Region of the United States | Virology Journal BioMed Central Research Phylogenetic analysis of Newcastle disease viruses isolated from waterfowl in the Upper Midwest Region of the United States Naresh Jindal Yogesh Chander Ashok K Chockalingam Martha de Abin Patrick T Redig and Sagar M Goyal Address Department of Veterinary Population Medicine College of Veterinary Medicine University of Minnesota 1333 Gortner Avenue Saint Paul MN 55108 USA Email Naresh Jindal - jinda014@ Yogesh Chander - chand062@ Ashok K Chockalingam - chock006@ Martha de Abin - fuent006@ Patrick T Redig - redig001@ Sagar M Goyal - goyal001@ Corresponding author Open Access Published 5 November 2009 Received 14 July 2009 Accepted 5 November 2009 Virology Journal 2009 6 191 doi 1743-422X-6-191 This article is available from http content 6 1 191 2009 Jindal et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background This study was conducted to characterize Newcastle disease virus NDV isolates obtained from waterfowl from the Upper Midwest region of the United States. A total of 43 NDVs were isolated by inoculation of cloacal samples in embryonated chicken eggs. These isolates were obtained from 24 mallards seven American green-winged teals six northern pintails four bluewinged teals and two wood ducks. Partial sequences of fusion gene were analyzed to determine the pathotypes and genotypes involved. Results Deduced amino acid sequence of the cleavage site of fusion F protein revealed that all isolates had avirulent motifs. Of the 43 isolates 23 exhibited sequence motif of 111GGKQGRL117 at the cleavage site 19 exhibited 111GEKQGRL117 while one isolate showed 111GERQGRL117. Phylogenetic analysis .

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