TAILIEUCHUNG - Báo cáo khoa học: "Molecular characterisation of virulence graded field isolates of myxoma virus"

Molecular characterisation of virulence graded field isolates of myxoma virus | Dalton et al. Virology Journal 2010 7 49 http content 7 1 49 VIROLOGY JOURNAL RESEARCH Open Access Molecular characterisation of virulence graded field isolates of myxoma virus Kevin P Dalton Ines Nicieza Aroa Baragano Jose Manuel Martín Alonso Francisco Parra Abstract Background Myxoma virus MV has been endemic in Europe since shortly after its deliberate release in France in 1952. While the emergence of more resistant hosts and more transmissible and attenuated virus is well documented there have been relatively few studies focused on the sequence changes incurred by the virus as it has adapted to its new host. In order to identify regions of variability within the MV genome to be used for phylogenetic studies and to try to investigate causes of MV strain attenuation we have molecularly characterised nine strains of MV isolated in Spain between the years 1992 and 1995 from wide ranging geographic locations and which had been previously graded for virulence by experimental infection of rabbits. Results The findings reported here show the analysis of 16 genomic regions accounting for approximately 10 of the viral genomes. Of the 20 genes analysed 5 M034L M069L M071L M130R and M135R were identical in all strains and 1 M122R contained only a single point mutation in an individual strain. Four genes M002L R M009L M036L and M017L showed insertions or deletions that led to disruption of the ORFs. Conclusions The findings presented here provide valuable tools for strain differentiation and phylogenetic studies of MV isolates and some clues as to the reasons for virus attenuation in the field. Background Myxoma virus MV causes myxomatosis in the European rabbit Oryctolagus cuniculus . The disease is a recurrent problem in rabbit farms and in wild rabbit populations throughout Europe 1-4 . Due to the unique manner of its introduction into the European rabbit population MV provides an excellent model for studying the coevolution of a virus and its host. .

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