TAILIEUCHUNG - Báo cáo khoa học: Transcriptional activity and Sp 1⁄3 transcription factor binding to the P1 promoter sequences of the human AbH-J-J locus

Alternative splicing of the locus AbH-J-J generates functionally distinct proteins: the enzyme aspartyl (asparaginyl) b-hydroxylase, humbug and junctate (truncated homologs of aspartyl (asparaginyl) b-hydroxylase with a role in calcium regulation), and junctin (a structural protein of the sarco-plasmic reticulum membrane). | ễFEBS Journal Transcriptional activity and Sp 1 3 transcription factor binding to the P1 promoter sequences of the human ApH-J-J locus Giordana Feriotto1 Alessia Finotti2 Giulia Breveglieri1 Susan Treves3 Francesco Zorzato4 and Roberto Gambari2 1 Biotechnology Center University of Ferrara Italy 2 Department of Biochemistry and Molecular Biology Section of Molecular Biology University of Ferrara Italy 3 Departments of Anaesthesia and Research Kantonsspital Basel Switzerland 4 Department of Experimentaland Diagnostic Medicine Section of GeneralPathology University of Ferrara Italy Keywords aspartyl asparaginyl b-hydroxylase humbug junctate specific transcription factors transcription Correspondence R. Gambari Department of Biochemistry and Molecular Biology University of Ferrara Via Borsari 46 44100 Ferrara Italy Fax 39 532 202723 Tel 39 532 424443 E-mail gam@ Received 22 May 2007 revised 29 June 2007 accepted 3 July 2007 doi Alternative splicing of the locus generates functionally distinct proteins the enzyme aspartyl asparaginyl b-hydroxylase humbug and junctate truncated homologs of aspartyl asparaginyl b-hydroxylase with a role in calcium regulation and junctin a structural protein of the sarcoplasmic reticulum membrane . Aspartyl asparaginyl b-hydroxylase and humbug are overexpressed in a broad range of malignant neoplasms. We have previously reported the gene structure of this locus showing the presence of two putative promoters P1 and P2 and characterized the P2 sequences directing tissue-specific transcription of junctin aspartyl aspar-aginyl b-hydroxylase and junctate. In addition aspartyl asparaginyl b-hydroxylase and humbug are expressed from exon 1 by the P1 promoter. The present study identifies and functionally characterizes the P1 promoter activity of the AbH-J-J locus. We demonstrate that mRNAs from the P1 promoter are actively transcribed in all the human tissues and cell lines analyzed and define .

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