TAILIEUCHUNG - Báo cáo y học: " The epitope of the VP1 protein of porcine parvovirus Short report"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The epitope of the VP1 protein of porcine parvovirus Short report | Xie et al. Virology Journal 2010 7 161 http content 7 1 161 VIROLOGY JOURNAL SHORT REPORT Open Access The epitope of the VP1 protein of porcine parvovirus Hong-ling Xie 1 2 Zhao Wang3 Shang-jin Cui 1 Chao-fan Zhang1 and Yu-dong Cui2 Abstract Porcine parvovirus PPV is the major causative agent in a syndrome of reproductive failure in swine. Much has been learned about the structure and function of PPV in recent years but nothing is known about the epitopes of the structural protein VP1 which is an important antigen of PPV. In this study the monoclonal antibody C4 against VP1 of PPV was prepared and was used to biopan a 12-mer phage peptide library three times. The selected phage clones were identified by ELISA and then sequencing. The amino acid sequences detected by phage display were analyzed and a mimic immuno-dominant epitope was identified. The epitope of VP1 is located in the N-terminal and contains the role amino acid sequence R-K-R. Immunization of mice indicated that the phage-displayed peptide induces antibodies against PPV. This study shows that peptide mimotopes have potential as alternatives to the complex antigens currently used for diagnosis of PPV infection or for development of vaccines. Introduction Porcine parvovirus PPV which was first isolated from sows in Germany by Mayr et al. 1 and which belongs to the genus Parvovirus in the family Parvoviridae is the major causative virus in a syndrome of reproductive failure in swine. This syndrome is referred to as SEDI and includes stillbirths mummified fetuses early embryonic death and infertility 2 . Recent studies indicate that in addition to inducing reproductive failure PPV also causes dermatitis diarrhea and respiratory system disease 3-10 . PPV is composed of structural protein and non-struc-tural protein and the structural protein is the virus s main immunological antigen. Using SDS-PAGE Moliter et al. 11 identified three kinds of structural proteins VP1 VP2 and VP3. All three .

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