TAILIEUCHUNG - Báo cáo y học: " EBNA3C interacts with Gadd34 and counteracts the unfolded protein response"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: EBNA3C interacts with Gadd34 and counteracts the unfolded protein response | Virology Journal BioMed Central Research EBNA3C interacts with Gadd34 and counteracts the unfolded protein response Jose L Garrido 1 Seijii Maruo2 Kenzo Takada2 and Adam Rosendorff1 Open Access Address University of Pittsburgh and Children s Hospital of Pittsburgh One Children s Hospital Drive 4401 Penn Ave Pittsburgh PA 15224 USA and 2Department of Tumor Virology Institute for Genetic Medicine Hokkaido University N15 W7 Kita-ku Sapporo 060-0815 Japan Email Jose L Garrido - jlg114@ Seijii Maruo - smaruo@ Kenzo Takada - kentaka@ Adam Rosendorff- Corresponding author Published 29 December 2009 Received 26 October 2009 Accepted 29 December 2009 Virology Journal 2009 6 231 doi 1743-422X-6-23 1 This article is available from http content 6 1 231 2009 Garrido et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract EBNA3C is an EBV-encoded nuclear protein essential for proliferation of EBV infected B-lymphocytes. Using EBNA3C amino acids 365-545 in a yeast two hybrid screen we found an interaction with the Growth Arrest and DNA-damage protein Gadd34. When both proteins are overexpressed Gadd34 can interact with EBNA3C in both nuclear and cytoplasmic compartments. Amino acids 483-610 of Gadd34 including the two PP1a interaction and the HSV-1 homology domains are required for the interaction. Furthermore interaction is lost with a mutant of EBNA3C 509 DVIEVID 515 AVIAVIA that abolishes EBNA3C coactivation ability as well as SUMO interaction 1 . In B-cells Gadd34 and EBNA3C are present in a complex with PP1a using microcystin sepharose affinity purification Using a lymphoblastoid cell line in which EBNA3C protein levels are .

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