TAILIEUCHUNG - Báo cáo khoa học: In vitro stimulatory effect of anti-apoptotic seminal vesicle protein 4 on purified peroxidase enzymes

The enzymatic activities of purified horseradish peroxidase, selenium-depen-dent glutathione peroxidase, thyroid peroxidase and myeloperoxidase, but not that of lactoperoxidase, were markedly enhanced when added into a reaction mixture containing 5lmnative seminal vesicle protein 4, a major protein secreted from rat seminal vesicle epithelium. | In vitro stimulatory effect of anti-apoptotic seminal vesicle protein 4 on purified peroxidase enzymes Vittoria Metafora1 Paola Stiuso2 Pasquale Ferranti3 Angela Giannattasio1 Alessandra Dicitore2 Gianpietro Ravagnan4 Salvatore De Maria5 Gabriele Pontoni2 Maria Cartenl5 and Salvatore Metafora1 1 Institute of Genetics and Biophysics Adriano Buzzati-Traverso CNR Naples Italy 2 Department of Biochemistry and Biophysics Medical School II University of Naples Italy 3 Department of Food Science University of Napoli Federico II Portici Italy 4 Department of EnvironmentalSciences Ca Foscari Venezia University Italy 5 Department of ExperimentalMedicine Medical School II University of Naples Italy Keywords anti-ROS cell protection enzymatic kinetics peroxidase stimulation protein SV-IV reactive oxygen species Correspondence S. Metafora CNR Institute of Genetics and Biophysics Adriano Buzzati Traverso Via Pietro Castellino 111 80128 Naples Italy Fax 39 0816132253 Tel 39 0816132254 E-mail metafora@ Received 26 March 2008 revised 29 May 2008 accepted 3 June 2008 doi The enzymatic activities of purified horseradish peroxidase selenium-dependent glutathione peroxidase thyroid peroxidase and myeloperoxidase but not that of lactoperoxidase were markedly enhanced when added into a reaction mixture containing 5 pM native seminal vesicle protein 4 a major protein secreted from rat seminal vesicle epithelium. A further increase of horseradish peroxidase activity was obtained using Ser58-phosphorylated or acetylated seminal vesicle protein 4. The activating effect of native seminal vesicle protein 4 was highest about 60-fold on horseradish peroxidase when 4-chloro-1-naphtol was used as the electron donor substrate. The main kinetics parameters of the stimulatory effect on horseradish peroxidase were evaluated and the enzyme-electron donor substrate interaction was investigated by HPLC and electrospray-MS. A native seminal vesicle protein 4 .

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