TAILIEUCHUNG - Báo cáo y học: " An efficient in vitro-inoculation method for Tomato yellow leaf curl virus"

An efficient in vitro-inoculation method for Tomato yellow leaf curl virus | Al Abdallat et al. Virology Journal 2010 7 84 http content 7 1 84 VIROLOGY JOURNAL RESEARCH Open Access An efficient in vitro-inoculation method for Tomato yellow leaf curl virus Ayed M Al Abdallat 1 2 Hmoud S Al Debei1 Heba Asmar1 Samar Misbeh1 Ayat Quraan1 and Anders Kvarnheden3 Abstract Background Tomato yellow leaf curl virus TYLCV is a member of the family Geminiviridae genus Begomovirus. To test the infectivity of TYLCV in tomato plants an improved protocol for inoculation of in vitro-cultured tomato plants was developed. Results A TYLCV isolate was cloned sequenced and used to construct a infectious clone. Three weeks old microshoots of TYLCV-susceptible tomato plants were inoculated with Agrobacterium tumefaciens harboring the infectious clone for the TYLCV isolate. After two weeks the TYLCV symptoms started to appear on the in vitro-inoculated plants and the symptoms became more severe and pronounced eight weeks post-inoculation. The method was used efficiently to uncover the resistance mechanism against TYLCV in Solanum habrochaites accession LA 1777 a wild tomato known for its high resistance to whitefly and TYLCV. Conclusions The reported in vitro-inoculation method can be used to screen tomato genotypes for their responses to TYLCV under controlled conditions and it will be a useful tool for better understanding of the TYLCV biology in tomato plants. Background Since it was first reported in the Jordan valley 1 the tomato yellow leaf curl disease TYLCD caused by the Tomato yellow leaf curl virus TYLCV and related viruses has become a serious problem that affects tomato Solanum lycopersicum worldwide. TYLCV is a member of the family Geminiviridae genus Begomovirus and it is transmitted to tomato by the whitefly Bemisia tabaci in a persistent and circulative manner 2 . The management of the disease in tomato production areas is difficult and expensive and the control measurements are focused mainly on the whitefly control and .

TÀI LIỆU LIÊN QUAN
TAILIEUCHUNG - Chia sẻ tài liệu không giới hạn
Địa chỉ : 444 Hoang Hoa Tham, Hanoi, Viet Nam
Website : tailieuchung.com
Email : tailieuchung20@gmail.com
Tailieuchung.com là thư viện tài liệu trực tuyến, nơi chia sẽ trao đổi hàng triệu tài liệu như luận văn đồ án, sách, giáo trình, đề thi.
Chúng tôi không chịu trách nhiệm liên quan đến các vấn đề bản quyền nội dung tài liệu được thành viên tự nguyện đăng tải lên, nếu phát hiện thấy tài liệu xấu hoặc tài liệu có bản quyền xin hãy email cho chúng tôi.
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.