TAILIEUCHUNG - Báo cáo khoa học: Incorporation of ZP1 into perivitelline membrane after in vivo treatment with exogenous ZP1 in Japanese quail (Coturnix japonica)

In birds, the egg envelope surrounding the oocyte prior to ovulation is called the perivitelline membrane and it plays important roles in fertiliza-tion. In a previous study we demonstrated that one of the components of the perivitelline membrane, ZP3, which is secreted from the ovarian granu-losa cells, specifically interacts with ZP1, another constituent that is synthe-sized in the liver of Japanese quail. | ễFEBS Journal Incorporation of ZP1 into perivitelline membrane after in vivo treatment with exogenous ZP1 in Japanese quail Coturnix japonica Mihoko Kinoshita1 Kaori Mizui1 Tsukasa Ishiguro1 Mamoru Ohtsuki1 Norio Kansaku2 Hiroshi Ogawa3 Akira Tsukada4 Tsukasa Sato1 and Tomohiro Sasanami1 1 Department of Applied BiologicalChemistry Faculty of Agriculture Shizuoka University Japan 2 Laboratory of AnimalGenetics and Breeding Azabu University Fuchinobe Sagamihara Japan 3 Laboratory of Wild Animals Tokyo University of Agriculture Atsugi-shi Japan 4 Graduate Schoolof BioagriculturalSciences Nagoya University Japan Keywords Japanese quail matrix assembly perivitelline membrane zona pellucida ZP1 Correspondence T. Sasanami Department of Applied BiologicalChemistry Faculty of Agriculture Shizuoka University 836 Ohya Shizuoka 422-8529 Japan Fax Tel 81 54 238 4526 E-mail atsasan@ Received 20 March 2008 revised 7 May 2008 accepted 13 May 2008 doi In birds the egg envelope surrounding the oocyte prior to ovulation is called the perivitelline membrane and it plays important roles in fertilization. In a previous study we demonstrated that one of the components of the perivitelline membrane ZP3 which is secreted from the ovarian granulosa cells specifically interacts with ZP1 another constituent that is synthesized in the liver of Japanese quail. In the present study we investigated whether ZP1 injected exogenously into the blood possesses the ability to reconstruct the perivitelline membrane of Japanese quail. When ZP1 purified from the serum of laying quail was injected into other female birds the signal of this exogenous ZP1 was detected in the perivitelline membrane. In addition we revealed by means of ligand blot analysis that serum ZP1 interacts with both ZP1 and ZP3 of the perivitelline membrane. By contrast when ZP1 derived from the perivitelline membrane was administered it failed to become incorporated into the .

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