TAILIEUCHUNG - Báo cáo y học: " Characterization and frequency of a newly identified HIV-1 BF1 intersubtype circulating recombinant form in São Paulo, Brazil"

Characterization and frequency of a newly identified HIV-1 BF1 intersubtype circulating recombinant form in São Paulo, Brazil | Sanabani et al. Virology Journal 2010 7 74 http content 7 1 74 VIROLOGY JOURNAL RESEARCH Open Access Characterization and frequency of a newly identified HIV-1 BF1 intersubtype circulating recombinant form in São Paulo Brazil 1 Sabri Saeed Sanabani Evelyn Regina de Souza Pastena Walter Kleine Neto Vanessa Pouza Martinez Ester Cerdeira Sabino1 Abstract Background HIV circulating recombinant forms CRFs play an important role in the global and regional HIV epidemics particularly in regions where multiple subtypes are circulating. To date several 40 CRFs are recognized worldwide with five currently circulating in Brazil. Here we report the characterization of near fulllength genome sequences NFLG of six phylogenetically related HIV-1 BF1 intersubtype recombinants five from this study and one from other published sequences representing CRF46_BF1. Methods Initially we selected 36 samples from 888 adult patients residing in São Paulo who had previously been diagnosed as being infected with subclade F1 based on pol subgenomic fragment sequencing. Proviral DNA integrated in peripheral blood mononuclear cells PBMC was amplified from the purified genomic DNA of all 36-blood samples by five overlapping PCR fragments followed by direct sequencing. Sequence data were obtained from the five fragments that showed identical genomic structure and phylogenetic trees were constructed and compared with previously published sequences. Genuine subclade F1 sequences and any other sequences that exhibited unique mosaic structures were omitted from further analysis Results Of the 36 samples analyzed only six sequences inferred from the pol region as subclade F1 displayed BF1 identical mosaic genomes with a single intersubtype breakpoint identified at the nef-U3 overlap HXB2 position 9347-9365 LTR region . Five of these isolates formed a rigid cluster in phylogentic trees from different subclade F1 fragment regions which we can now designate as CRF46_BF1. .

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