TAILIEUCHUNG - báo cáo khoa học: " QTLs and candidate genes for desiccation and abscisic acid content in maize kernels"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: QTLs and candidate genes for desiccation and abscisic acid content in maize kernels | Capelle et al. BMC Plant Biology 2010 10 2 http 1471-2229 10 2 BMC Plant Biology RESEARCH ARTICLE Open Access QTLs and candidate genes for desiccation and abscisic acid content in maize kernels Valérie Capelle1 2 Carine Remoué2 3 Laurence Moreau4 Agnès Reyss1 2 Aline Mahé1 2 Agnès Massonneau5 6 í iioi I pair ll IQ4 Ạ I in rrriccot4 r l I Hino THó anr 1 1 2 Dcitcir Dr tr irvA cl x 5 r i ircr J4 lo n-l 1 lie Drib ll 1 2 Matthieu Falque Alain Charcosset Claudine Thévenot Peter Rogowsky Sylvie Coursol Jean-Louis Prioul Abstract Background Kernel moisture at harvest is an important trait since a low value is required to prevent unexpected early germination and ensure seed preservation. It is also well known that early germination occurs in viviparous mutants which are impaired in abscisic acid ABA biosynthesis. To provide some insight into the genetic determinism of kernel desiccation in maize quantitative trait loci QTLs were detected for traits related to kernel moisture and ABA content in both embryo and endosperm during kernel desiccation. In parallel the expression and mapping of genes involved in kernel desiccation and ABA biosynthesis were examined to detect candidate genes. Results The use of an intermated recombinant inbred line population allowed for precise QTL mapping. For 29 traits examined in an unreplicated time course trial of days after pollination a total of 78 QTLs were detected 43 being related to kernel desiccation 15 to kernel weight and 20 to ABA content. Multi QTL models explained 35 to 50 of the phenotypic variation for traits related to water status indicating a large genetic control amenable to breeding. Ten of the 20 loci controlling ABA content colocated with previously detected QTLs controlling water status and ABA content in water stressed leaves. Mapping of candidate genes associated with kernel desiccation and ABA biosynthesis revealed several colocations between genes with putative functions and QTLs. Parallel .

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