TAILIEUCHUNG - báo cáo khoa học: " Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites | Dalal et al. BMC Plant Biology 2010 10 61 http 1471-2229 10 61 BMC Plant Biology RESEARCH ARTICLE _ Open Access Isolation and functional characterization of Lycopene -cyclase CYC-B promoter from Solanum habrochaites Monika Dalal 1 2 Viswanathan Chinnusamy3 and Kailash C Bansal 1 Abstract Background Carotenoids are a group of C40 isoprenoid molecules that play diverse biological and ecological roles in plants. Tomato is an important vegetable in human diet and provides the vitamin A precursor fi-carotene. Genes encoding enzymes involved in carotenoid biosynthetic pathway have been cloned. However regulation of genes involved in carotenoid biosynthetic pathway and accumulation of specific carotenoid in chromoplasts are not well understood. One of the approaches to understand regulation of carotenoid metabolism is to characterize the promoters of genes encoding proteins involved in carotenoid metabolism. Lycopene fi-cyclase is one of the crucial enzymes in carotenoid biosynthesis pathway in plants. Its activity is required for synthesis of both a-and p-carotenes that are further converted into other carotenoids such as lutein zeaxanthin etc. This study describes the isolation and characterization of chromoplast-specific Lycopene fi-cyclase CYC-B promoter from a green fruited S. habrochaites genotype EC520061. Results A 908 bp region upstream to the initiation codon of the Lycopene fi-cyclase gene was cloned and identified as full-length promoter. To identify promoter region necessary for regulating developmental expression of the ShCYC-B gene the full-length promoter and its three different 5 truncated fragments were cloned upstream to the initiation codon of GUS reporter cDNA in binary vectors. These four plant transformation vectors were separately transformed in to Agrobacterium. Agrobacterium-mediated transient and stable expression systems were used to study the GUS expression driven by the full-length promoter and its 5 deletion fragments .

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