TAILIEUCHUNG - Báo cáo khoa học: Structural evidence of a-aminoacylated lipoproteins of Staphylococcus aureus

Bacterial lipoproteins are known to be diacylated or triacylated and acti-vate mammalian immune cells via Toll-like receptor 2⁄6or2⁄1 heterodi-mer. Because the genomes of low G+C content Gram-positive bacteria, such as Staphylococcus aureus, do not contain Escherichia coli-type apoli-poproteinN-acyltransferase | 1FEBS Journal Structural evidence of a-aminoacylated lipoproteins of Staphylococcus aureus Miwako Asanuma1 z Kenji Kurokawa2 Rie Ichikawa1 Kyoung-Hwa Ryu2 Jun-Ho Chae2 Naoshi Dohmae1 Bok Luel Lee2 and Hiroshi Nakayama1 1 Biomolecular Characterization Team RIKEN Advanced Science Institute Saitama Japan 2 NationalResearch Laboratory of Defense Proteins Pusan NationalUniversity Busan Korea Keywords bacteriallipoprotein Gram-positive bacteria mass spectrometry N-acyltransferase SitC Correspondence B. L. Lee National Research Laboratory of Defense Proteins College of Pharmacy Pusan NationalUniversity Jangjeon Dong Geumjeong Gu Busan 609-735 Korea Fax 82 51 513 2801 Tel 82 51 510 2809 E-mail brlee@ H. Nakayama Biomolecular Characterization Team RIKEN Advanced Science Institute 2-1 Hirosawa Wako Saitama 351-0198 Japan Fax 81 48 462 4704 Tel 81 48 462 1419 E-mail knife@ Present address ERATO Japan Science and Technology Agency JST Tokyo Japan Received 12 November 2010 revised 3 December 2010 accepted 9 December 2010 doi Bacterial lipoproteins are known to be diacylated or triacylated and activate mammalian immune cells via Toll-like receptor 2 6 or 2 1 heterodimer. Because the genomes of low G C content Gram-positive bacteria such as Staphylococcus aureus do not contain Escherichia coli-type apolipoprotein N-acyltransferase an enzyme converting diacylated lipoproteins into triacylated forms it has been widely believed that native lipoproteins of S. aureus are diacylated. However we recently demonstrated that one lipoprotein Site purified from S. aureus RN4220 strain was triacylated. Almost simultaneously another group reported that another lipoprotein SA2202 purified from S. aureus SA113 strain was diacylated. The determination of exact lipidated structures of S. aureus lipoproteins is thus crucial for elucidating the molecular basis of host-microorganism interactions. Toward this purpose we intensively used MS-based .

• Báo cáo khoa học
• Scientific reports
• breast cancer
• medicine
• Staphylococcus aureus
• aminoacylated lipoproteins
• medical research
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes